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Literature summary extracted from

  • Nevins, J.R.; Joklik, W.K.
    Isolation and partial characterization of the poly(A) polymerases from HeLa cells infected with vaccinia virus (1977), J. Biol. Chem., 252, 6939-6947.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.19 cytoplasm infected cytoplasm of HeLa cells Homo sapiens 5737
-
2.7.7.19 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 Mg2+ HeLa cells contain one enzyme form that is stimulated by Mn2+ and also by Mg2+, and a second one that is absolutely dependent on the presence of Mg2+ Homo sapiens
2.7.7.19 Mg2+ Vaccinia virus enzyme is stimulated by Mn2+ and also by Mg2+ Vaccinia virus
2.7.7.19 Mn2+ HeLa cells contain one enzyme form that is stimulated by Mn2+ and also by Mg2+, and a second one that is absolutely dependent on the presence of Mg2+ Homo sapiens
2.7.7.19 Mn2+ Vaccinia virus enzyme is stimulated by Mn2+ and also by Mg2+ Vaccinia virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.19 37000
-
 1 * 37000 + 1 * 57000, SDS-PAGE Vaccinia virus
2.7.7.19 50000
-
 1 * 50000, nuclear Mn2+- and Mg2+-activated enzyme, SDS-PAGE Homo sapiens
2.7.7.19 57000
-
 1 * 37000 + 1 * 57000, SDS-PAGE Vaccinia virus
2.7.7.19 58000
-
 nuclear Mg2+- and Mn2+-stimulated enzyme, glycerol density gradient sedimentation Homo sapiens
2.7.7.19 63000
-
 cytoplasmic Mn2+-dependent enzyme, glycerol density gradient sedimentation Homo sapiens
2.7.7.19 70000
-
 enzyme from infected cytoplasm, glycerol density gradient sedimentation Vaccinia virus
2.7.7.19 75000
-
 1 * 75000, cytoplasmic, Mn2+-dependent enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Homo sapiens
-
 HeLa infected with vaccinia virus
-
2.7.7.19 Homo sapiens
-
 2 forms: 1. nuclear enzyme, stimulated by Mn2+ and Mg2+, 2. cytoplasmic, dependent on Mn2+
-
2.7.7.19 Vaccinia virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19
-
 Homo sapiens
2.7.7.19
-
 Vaccinia virus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.19 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA primer required Homo sapiens diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA primer required Vaccinia virus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA human nuclear enzyme and Vaccinia virus enzyme are able to use both RNA and oligo(A) as primer, human cytoplasmic enzyme is able to use RNA but not oligo(A) Homo sapiens diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA human nuclear enzyme and Vaccinia virus enzyme are able to use both RNA and oligo(A) as primer, human cytoplasmic enzyme is able to use RNA but not oligo(A) Vaccinia virus diphosphate + RNA(A)n
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.19 dimer 1 * 37000 + 1 * 57000, SDS-PAGE Vaccinia virus
2.7.7.19 monomer 1 * 75000, cytoplasmic, Mn2+-dependent enzyme, SDS-PAGE Homo sapiens
2.7.7.19 monomer 1 * 50000, nuclear Mn2+- and Mg2+-activated enzyme, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.19 8
-
 vaccinia virus enzyme, human, cytoplasmic Mn2+-dependent enzyme Homo sapiens
2.7.7.19 8
-
 vaccinia virus enzyme, human, cytoplasmic Mn2+-dependent enzyme Vaccinia virus
2.7.7.19 8.3
-
 human nuclear Mn2+- and Mg2+-activated enzyme Homo sapiens