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Literature summary extracted from

  • Rose, K.M.; Jacob, S.T.
    Nuclear poly(A) polymerase from rat liver and a hepatoma. Comparison of properties, molecular weights and amino acid compositions (1976), Eur. J. Biochem., 67, 11-21.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.19 alpha-Amanitin
-
 Rattus norvegicus
2.7.7.19 ATP hepatoma enzyme less effective to substrate inhibition than liver enzyme Rattus norvegicus
2.7.7.19 Cordycepin 5'-triphosphate
-
 Rattus norvegicus
2.7.7.19 diphosphate
-
 Rattus norvegicus
2.7.7.19 K+ KCl; maximal stimulation at 40 mM, inhibition above 250 mM Rattus norvegicus
2.7.7.19 N-ethylmaleimide
-
 Rattus norvegicus
2.7.7.19 Pancreatic ribonuclease
-
 Rattus norvegicus
2.7.7.19 Rose bengal
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.19 0.07
-
 ATP 37°C, pH 8.0 Rattus norvegicus
2.7.7.19 0.15
-
 ATP 37°C, pH 8.0 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.19 nucleus
-
 Rattus norvegicus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 KCl maximal stimulation at 40 mM, inhibition above 250 mM Rattus norvegicus
2.7.7.19 Mg2+ 10% of the activity with Mn2+ Rattus norvegicus
2.7.7.19 Mn2+ absolute requirement Rattus norvegicus
2.7.7.19 Mn2+ optimal concentration: 0.25-0.75 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.19 48000
-
 x * 48000, liver enzyme, SDS-PAGE Rattus norvegicus
2.7.7.19 60000
-
 x * 60000, hepatoma enzyme, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.19 hepatoma cell Morris hepatoma tumor cells 3924A Rattus norvegicus
-
2.7.7.19 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.19 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA highly specific for ATP Rattus norvegicus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA primer required Rattus norvegicus diphosphate + RNA(A)n
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.19 ? x * 60000, hepatoma enzyme, SDS-PAGE Rattus norvegicus
2.7.7.19 ? x * 48000, liver enzyme, SDS-PAGE Rattus norvegicus