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Literature summary extracted from

  • Tsiapalis, C.M.; Dorson, J.W.; Bollum, F.J.
    Purification of terminal riboadenylate transferase from calf thymus gland (1975), J. Biol. Chem., 250, 4486-4496.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.19 freezing and thawing accelerates inactivation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.19 diphosphate
-
 Bos taurus
2.7.7.19 additional information insensitive to high levels of RNA-polymerase inhibitors Bos taurus
2.7.7.19 Na2HPO4
-
 Bos taurus
2.7.7.19 Ribonucleoside triphosphates other than ATP
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.19 0.05
-
 ATP oligoadenylate (in presence of Mn2+) Bos taurus
2.7.7.19 0.2
-
 oligoadenylate in presence of Mg2+, pH 9.0, 35°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.19 cytoplasm
-
 Bos taurus 5737
-
2.7.7.19 soluble
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 Mn2+ required Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.19 62000
-
 sucrose density gradient sedimentation, gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19 calf Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.19 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.19 28.33
-
 35°C, pH 9.0 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.7.19 -20°C, 50% glycerol, 80% inactivation in the first few weeks, the 20% remaining activity is stable for more than 2 years Bos taurus
2.7.7.19 -70°C or under liquid nitrogen, extensive inactivation Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + oligoadenylate
-
 Bos taurus ?
-
 ?
2.7.7.19 ATP + RNA highly specific for ATP Bos taurus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA primer required Bos taurus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA primer: variety of oligoribonucleotides having free 3'-OH Bos taurus diphosphate + RNA(A)n
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.19 monomer 1 * 62000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.19 35
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.19 30
-
 ATP cosubstrates rA(pA)5, pH 9.0, 35°C Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.19 8.3
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.19 7 8.8 about 50% of maximum activity at pH 7.0 and pH 8.8 Bos taurus