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Literature summary extracted from

  • Moss, B.; Rosenblum, E.N.; Gershowitz, A.
    Characterization of a polyriboadenylate polymerase from vaccinia virions (1975), J. Biol. Chem., 250, 4722-4729.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.19 Na+ 0.1 M; NaCl Vaccinia virus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.19 viral nucleocapsid
-
 Vaccinia virus 19013
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 Mg2+ more active in presence of Mn2+ than Mg2+ Vaccinia virus
2.7.7.19 Mn2+ more active in presence of Mn2+ than Mg2+ Vaccinia virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.19 35000
-
 1 * 51000 + 1 * 35000, SDS-PAGE Vaccinia virus
2.7.7.19 51000
-
 1 * 51000 + 1 * 35000, SDS-PAGE Vaccinia virus
2.7.7.19 80000
-
 sucrose density gradient sedimentation Vaccinia virus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Vaccinia virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19
-
 Vaccinia virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA highly specific for ATP Vaccinia virus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA primer required Vaccinia virus diphosphate + RNA(A)n
-
 ?
2.7.7.19 ATP + RNA minimum effective primer length is 4 to 6 nucleotides Vaccinia virus diphosphate + RNA(A)n
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.19 dimer 1 * 51000 + 1 * 35000, SDS-PAGE Vaccinia virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.19 8.6
-
-
 Vaccinia virus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.19 8 10 pH 8: about 40% of maximum activity, pH 10: about 50% of maximum activity Vaccinia virus