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Literature summary extracted from

  • Yang, W.; Boggs, K.P.; Jackowski, S.
    The association of lipid activators with the amphipathic helical domain of CTP:phosphocholine cytidylyltransferase accelerates catalysis by increasing the affinity of the enzyme for CTP (1995), J. Biol. Chem., 270, 23951-23957.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.15 wild-type and deletion mutants delta312-367, delta231-367 and delta257-367 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.15 additional information DELTA delta257-367 exhibits significantly lower specific activity and can not be activated by lipid, deletion mutant DELTA231-367 can not be activated by lipid Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.15 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphatidylcholine ET-18-OCH3, non-hydrolyzable lysophosphatidylcholine analog, competitive with respect to the lipid activator Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.5
-
 CTP delta312-367 mutant, presence of lipid, pH 6.5, 37°C Rattus norvegicus
2.7.7.15 0.7
-
 CTP wild-type, presence of lipid, pH 6.5, 37°C Rattus norvegicus
2.7.7.15 13.2
-
 CTP DELTA257-367 mutant, pH 6.5, 37°C Rattus norvegicus
2.7.7.15 13.9
-
 CTP DELTA312-367 mutant, absence of lipid, pH 6.5, 37°C Rattus norvegicus
2.7.7.15 19.2
-
 CTP DELTA231-367 mutant, pH 6.5, 37°C Rattus norvegicus
2.7.7.15 24.7
-
 CTP wild-type, absence of lipid, pH 6.5, 37°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
 Rattus norvegicus diphosphate + CDPcholine
-
 ?