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Literature summary extracted from

  • Helmink, B.A.; Braker, J.D.; Kent, C.; Friesen, J.A.
    Identification of lysine 122 and arginine 196 as important functional residues of rat CTP:phosphocholine cytidylyltransferase a (2003), Biochemistry, 42, 5043-5051.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.15
-
 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.15 K122A decreased Vmax Rattus norvegicus
2.7.7.15 K122R decreased Vmax Rattus norvegicus
2.7.7.15 R196K decreased Vmax, 5fold increased Km for phosphorylcholine, 23fold increased Km for CTP Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.45
-
 phosphocholine wild-type, pH 7, 37°C Rattus norvegicus
2.7.7.15 1.54
-
 CTP wild-type, pH 7, 37°C Rattus norvegicus
2.7.7.15 2.09
-
 CTP K122A mutant, pH 7, 37°C Rattus norvegicus
2.7.7.15 2.35
-
 phosphocholine R196K mutant, pH 7, 37°C Rattus norvegicus
2.7.7.15 4.78
-
 CTP K122R mutant, pH 7, 37°C Rattus norvegicus
2.7.7.15 35.7
-
 CTP R196K mutant, pH 7, 37°C Rattus norvegicus
2.7.7.15 36
-
 phosphocholine K122R mutant, pH 7, 37°C Rattus norvegicus
2.7.7.15 212
-
 phosphocholine K122A mutant, pH 7, 37°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.15 CTP + phosphocholine = diphosphate + CDP-choline catalytic mechanism Rattus norvegicus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
 Rattus norvegicus diphosphate + CDPcholine
-
 ?