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Literature summary extracted from

  • Friesen, J.A.; Campbell, H.A.; Kent, C.
    Enzymic and cellular characterization of a catalytic fragment of CTP:phosphocholine cytidylyltransferase a (1999), J. Biol. Chem., 274, 13384-13389.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.15 deletion mutant which is truncated after residue 236, called CCTalpha236 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.15 additional information deletion mutant which is truncated after residue 236, called CCTalpha236, independent of lipids, 50fold increase in activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.65
-
 phosphocholine wild-type, presence of lipid Rattus norvegicus
2.7.7.15 0.9
-
 phosphocholine CCTalpha236 mutant, absence of lipid Rattus norvegicus
2.7.7.15 0.97
-
 phosphocholine CCTalpha236 mutant, presence of lipid Rattus norvegicus
2.7.7.15 1.05
-
 phosphocholine wild-type, absence of lipid Rattus norvegicus
2.7.7.15 1.29
-
 CTP wild-type, presence of lipid Rattus norvegicus
2.7.7.15 1.69
-
 CTP wild-type, absence of lipid Rattus norvegicus
2.7.7.15 3.15
-
 CTP CCTalpha236 mutant, absence of lipid Rattus norvegicus
2.7.7.15 4.47
-
 CTP CCTalpha236 mutant, presence of lipid Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.15 50300
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.15 CTP + choline phosphate Rattus norvegicus membrane binding segment has important role in cellular lipid metabolism diphosphate + CDPcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15 deletion mutant which is truncated after residue 236, called CCTalpha236 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.15 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
 Rattus norvegicus diphosphate + CDPcholine
-
 ?
2.7.7.15 CTP + choline phosphate membrane binding segment has important role in cellular lipid metabolism Rattus norvegicus diphosphate + CDPcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.15 dimer homodimer, CCTalpha236 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.15 0.21
-
 phosphocholine wild-type, absence of lipid Rattus norvegicus
2.7.7.15 11.1
-
 phosphocholine CCTalpha236 mutant, presence of lipid Rattus norvegicus
2.7.7.15 12.6
-
 phosphocholine CCTalpha236 mutant, absence of lipid Rattus norvegicus
2.7.7.15 17.7
-
 phosphocholine wild-type, presence of lipid Rattus norvegicus