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Literature summary extracted from

  • Weinhold, P.A.; Feldman, D.A.
    Choline-phosphate cytidylyltransferase (1992), Methods Enzymol., 209, 248-258.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.15
-
 Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.7.15 removal of detergents leads to rapid loss of activity and self-aggregation, high tendency to bind to glass and plastic surfaces even in the presence of detergents Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.15 4-chloromercuribenzoate 80% inhibition at 0.0006 mM Rattus norvegicus
2.7.7.15 5,5'-dithiobis(2-nitrobenzoate)
-
 Rattus norvegicus
2.7.7.15 N-ethylmaleimide 80% inhibition at 1.5 mM; CTP and phosphocholine protect Rattus norvegicus
2.7.7.15 phosphate maximal inhibition of 60% at 120 mM Rattus norvegicus
2.7.7.15 Sphingolipids reversed by activating phospholipids Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.15 0.22
-
 CTP pH 7, 37°C Rattus norvegicus
2.7.7.15 0.24
-
 phosphocholine pH 7, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.15 cytosol
-
 Rattus norvegicus 5829
-
2.7.7.15 membrane
-
 Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.15 44500
-
 2 * 44500, SDS-PAGE Rattus norvegicus
2.7.7.15 97000
-
 gel filtration, glycerol density gradient centrifugation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.15 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.15
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.15 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.15 47.5
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.7.15 -70°C, 50 mM Tris-HCl, 150 mM NaCl, 1.0 mM EDTA, 2.0 mM DTT, 0.025% NaN3, pH 7.4, 0.03% Triton X-100, 200 mM phosphate, stable for several months Rattus norvegicus
2.7.7.15 4°C, 20% loss of activity per day Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.15 CTP + choline phosphate
-
 Rattus norvegicus diphosphate + CDPcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.15 dimer 2 * 44500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.15 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.15 7
-
-
 Rattus norvegicus