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Literature summary extracted from

  • Ning, B.; Elbein, A.D.
    Purification and properties of mycobacterial GDP-mannose pyrophosphorylase (1999), Arch. Biochem. Biophys., 362, 339-345.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.13 GDP-glucose 2.5 mM, 50% inhibition Mycolicibacterium smegmatis
2.7.7.13 glucose 1-phosphate 10 mM, 50% inhibition Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.113
-
 GTP pH 7.5, 37°C, cosubstrate mannose 1-phosphate Mycolicibacterium smegmatis
2.7.7.13 0.14
-
 GDPmannose pH 7.5, 37°C, cosubstrate diphosphate Mycolicibacterium smegmatis
2.7.7.13 0.168
-
 alpha-D-mannose 1-phosphate pH 7.5, 37°C, cosubstrate GTP Mycolicibacterium smegmatis
2.7.7.13 0.356
-
 diphosphate pH 7.5, 37°C, cosubstrate GDPmannose Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.13 soluble
-
 Mycolicibacterium smegmatis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Co2+ divalent cation required for activity, second best activator, maximal activation at 5 mM Mycolicibacterium smegmatis
2.7.7.13 Mg2+ divalent cation required for activity, best metal activator Mg2+, maximal activation at 5-10 mM Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 37000
-
 2 * 37000, SDS-PAGE Mycolicibacterium smegmatis
2.7.7.13 70000
-
 gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Mycolicibacterium smegmatis
-
 GDPmannose + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 DEAE-52, ammonium sulfate, propyl-agarose, methyl-agrose, Sephadex G-200, hydroxyapatite Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.13 2.99
-
-
 Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
2.7.7.13 0°C, 1 week, 50% loss of activity Mycolicibacterium smegmatis
2.7.7.13 frozen, several months, little loss of activity Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Mycolicibacterium smegmatis GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate no activity with glucose 1-phosphate, UTP, ATP and CTP Mycolicibacterium smegmatis GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Mycolicibacterium smegmatis diphosphate + GDP-mannose
-
 ?
2.7.7.13 ITP + alpha-D-mannose 1-phosphate 15-20% of activity with GTP Mycolicibacterium smegmatis IDPmannose + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.13 dimer 2 * 37000, SDS-PAGE Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 8
-
-
 Mycolicibacterium smegmatis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.13 5.5 9.5
-
 Mycolicibacterium smegmatis