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Literature summary extracted from

  • Szumilo, T.; Drake, R.R.; York, J.L.; Elbein, A.D.
    GDP-mannose pyrophosphorylase. Purification to homogeneity, properties, and utilization to prepare photoaffinity analogs (1993), J. Biol. Chem., 268, 17943-17950.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.13 Cu2+
-
 Sus scrofa
2.7.7.13 GDP 0.4 mM, 50% inhibition Sus scrofa
2.7.7.13 GDP-glucose inhibition of GDP-mannose phosphorolysis Sus scrofa
2.7.7.13 GDP-mannose noncompetitive inhibition of GDP-glucose phosphorolysis Sus scrofa
2.7.7.13 glucose 1-phosphate inhibition of GDP-mannose phosphorolysis Sus scrofa
2.7.7.13 GMP 0.4 mM, 50% inhibition Sus scrofa
2.7.7.13 Hg2+
-
 Sus scrofa
2.7.7.13 mannose 1-phosphate noncompetitive inhibition of GDP-glucose phosphorolysis Sus scrofa
2.7.7.13 Zn2+
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.02
-
 GDPmannose pH 7.5, 37°C Sus scrofa
2.7.7.13 0.1
-
 GTP pH 7.5, 37°C, cosubstrate mannose 1-phosphate Sus scrofa
2.7.7.13 0.13
-
 mannose 1-phosphate pH 7.5, 37°C, cosubstrate GTP Sus scrofa
2.7.7.13 0.33
-
 diphosphate pH 7.5, 37°C, cosubstrate GDPglucose Sus scrofa
2.7.7.13 0.5
-
 diphosphate pH 7.5, 37°C, cosubstrate GDPmannose Sus scrofa
2.7.7.13 1
-
 GTP pH 7.5, 37°C, cosubstrate glucose 1-phosphate Sus scrofa
2.7.7.13 2.85
-
 glucose 1-phosphate pH 7.5, 37°C, cosubstrate GTP Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.13 Co2+ slight activation at about 10 mM Sus scrofa
2.7.7.13 Mg2+ maximal activation at 5 mM Sus scrofa
2.7.7.13 Mn2+ 70% of activity with Mg2+, inhibition at 10 mM and higher Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.13 37000
-
 x * 37000 + x * 43000 SDS-PAGE Sus scrofa
2.7.7.13 43000
-
 x * 37000 + x * 43000 SDS-PAGE Sus scrofa
2.7.7.13 450000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Sus scrofa
-
 GDPmannose + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 acid treatment, protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-200, hydroxylapatite, Blue Sepharose Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.13 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.13 9.25
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.7.13 frozen, 20 mM Tris-HCl, pH 7.5, 1 mM 2-mercaptoethanol, 1 mM EDTA, several months, no loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 8-Azido-GTP + glucose 1-phosphate
-
 Sus scrofa 8-Azido-GDPglucose + diphosphate
-
 ?
2.7.7.13 8-Azido-GTP + mannose 1-phosphate
-
 Sus scrofa 8-Azido-GDP mannose + diphosphate
-
 ?
2.7.7.13 GDP-glucose + diphosphate most effective substrate in direction of nucleoside triphosphate formation, in the reverse direction GTP is a better glucose acceptor than ITP Sus scrofa D-glucose 1-phosphate + GTP
-
 r
2.7.7.13 GDPmannose + diphosphate 61% of the activity with GDPglucose Sus scrofa ?
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Sus scrofa GDPmannose + diphosphate
-
 r
2.7.7.13 IDPmannose + diphosphate 72% of activity with GDPglucose Sus scrofa ITP + mannose 1-phosphate
-
 r
2.7.7.13 ITP + alpha-D-mannose 1-phosphate ITP is more effective than GTP with mannose 1-phosphate Sus scrofa IDPmannose + diphosphate
-
 r
2.7.7.13 ITP + glucose 1-phosphate
-
 Sus scrofa IDP-glucose + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.13 ? x * 37000 + x * 43000 SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.13 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 5.5 7.5 GTP synthesis Sus scrofa
2.7.7.13 6 8.5 GDPmannose synthesis Sus scrofa
2.7.7.13 8.2
-
 assay at Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.13 6 7.7 most stable at Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.13 0.024
-
 GDP-mannose pH 7.5, 37°C Sus scrofa
2.7.7.13 0.06
-
 mannose 1-phosphate pH 7.5, 37°C Sus scrofa
2.7.7.13 2.5
-
 glucose 1-phosphate pH 7.5, 37°C Sus scrofa
2.7.7.13 11
-
 GDP-glucose pH 7.5, 37°C Sus scrofa