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Literature summary extracted from

  • Crews, C.; Wilkinson, K.D.; Wells, L.; Perkins, C.; Fridovich-Keil, J.L.
    Functional consequence of substitutions at residue 171 in human galactose-1-phosphate uridylyltransferase (2000), J. Biol. Chem., 275, 22847-22853.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.12 expression of wild-type and mutants in Saccharomyces cerevisiae Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.12 F171L site-directed mutagenesis, 10fold decreased activity Homo sapiens
2.7.7.12 F171S site-directed mutagenesis, no activity Homo sapiens
2.7.7.12 F171W site-directed mutagenesis, severely reduced abundance Homo sapiens
2.7.7.12 F171Y site-directed mutagenesis, 4% activity compared to wild-type, no inhibition by excess UDP-glucose Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.12 UDP-glucose at high concentrations, wild-type and mutants F171L and F171S, not F171Y Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.12 additional information
-
 additional information kinetics Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Homo sapiens
-
 native mutant F171S
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 His-tagged recombinant wild-type and mutants F171Y and F171L to near homogeneity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate
-
 Homo sapiens alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.12 More modeling of the human enzyme structure onto the crystal structure of the Escherichia coli enzyme Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.7.12 GALT
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.12 3.6
-
 alpha-D-galactose 1-phosphate recombinant wild-type, pH 8.7 Homo sapiens
2.7.7.12 9.3
-
 alpha-D-galactose 1-phosphate recombinant wild-type, pH 8.7 Homo sapiens
2.7.7.12 98
-
 alpha-D-galactose 1-phosphate recombinant wild-type, pH 8.7 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.12 8.7
-
 assay at Homo sapiens