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Literature summary extracted from

  • Ruzicka, F.J.; Wedekind, J.E.; Kim, J.; Rayment, I.; Frey, P.A.
    Galactose-1-phosphate uridylyltransferase from Escherichia coli, a zinc and iron metalloenzyme (1995), Biochemistry, 34, 5610-5617.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.12 overexpression in strain BL21 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.12 1,10-phenanthroline inactivation of the enzyme by chelation of Zn2+ and Fe2+ ions, UDP-glucose protects, but alpha-D-glucose 1-phosphate does not Escherichia coli
2.7.7.12 2,2'-bipyridyl inactivation of the enzyme by chelation of Zn2+ and Fe2+ ions Escherichia coli
2.7.7.12 8-hydroxyquinoline inactivation of the enzyme by chelation of Zn2+ and Fe2+ ions Escherichia coli
2.7.7.12 8-Hydroxyquinoline sulfonate inactivation of the enzyme by chelation of Zn2+ and Fe2+ ions Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.12 Fe2+ required Escherichia coli
2.7.7.12 Fe2+ 0.7 mol per mol of subunit Escherichia coli
2.7.7.12 additional information enzyme contains no Ca, Cd, Cu, Mo, Ni, Co, Mn, As, Pb, or Se Escherichia coli
2.7.7.12 Zn2+ required Escherichia coli
2.7.7.12 Zn2+ 1.2 mol tightly bound per mol of subunit Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 recombinant from overexpressing strain BL21(DE3) Escherichia coli
2.7.7.12 to homogeneity Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.7.12 enzyme reconstitution with different metal ions after denaturation with urea to eliminate the natively bound metal ions Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.12 170 190 purified recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate
-
 Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
 r