Literature summary extracted from

Segawa, T.; Fukasawa, T.
The enzymes of the galactose cluster in Saccharomyces cerevisiae. Purification and characterization of galactose-1-phosphate uridylyltransferase (1979), J. Biol. Chem., 254, 10707-10709.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.7.12	0.2	0.26	UDP-glucose	pH 8.7, 25°C	Saccharomyces cerevisiae
2.7.7.12	4	-	alpha-D-galactose 1-phosphate	pH 8.7, 25°C	Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.7.12	38000	-	2 * 38000, SDS-PAGE	Saccharomyces cerevisiae
2.7.7.12	86100	-	sedimentation equilibrium centrifugation	Saccharomyces cerevisiae

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.12	Saccharomyces cerevisiae	-	-	-
2.7.7.12	Saccharomyces cerevisiae 106-3D	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.12	to homogeneity	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.7.12	54.1	-	purified enzyme	Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.7.12	UDP-glucose + alpha-D-galactose 1-phosphate	-	Saccharomyces cerevisiae	alpha-D-glucose 1-phosphate + UDP-galactose	-	r
2.7.7.12	UDP-glucose + alpha-D-galactose 1-phosphate	-	Saccharomyces cerevisiae 106-3D	alpha-D-glucose 1-phosphate + UDP-galactose	-	r

Subunits

EC Number	Subunits	Comment	Organism
2.7.7.12	dimer	2 * 38000, SDS-PAGE	Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.7.7.12	27	-	assay at	Saccharomyces cerevisiae

Temperature Range [°C]

EC Number	Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
2.7.7.12	additional information	-	activation energy, temperature profile	Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.7.7.12	987	-	alpha-D-galactose 1-phosphate	pH 8.7, 25°C	Saccharomyces cerevisiae

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.7.7.12	8.4	8.8	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)