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Literature summary extracted from

  • Lee, L.; Kimura, A.; Tochikura, T.
    Purification and properties of hexose 1-phosphate uridylyltransferase from Bifidobacterium bifidum (1978), Agric. Biol. Chem., 42, 723-730.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.7.12 glycerol 20% stabilizes Bifidobacterium bifidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.12 HgCl2 94% inhibition at 0.2 mM Bifidobacterium bifidum
2.7.7.12 iodoacetate no inhibition Bifidobacterium bifidum
2.7.7.12 additional information no inhibition by NaF Bifidobacterium bifidum
2.7.7.12 NEM no inhibition Bifidobacterium bifidum
2.7.7.12 PCMB 42% inhibition at 0.2 mM Bifidobacterium bifidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.12 0.023
-
 UDP-glucose pH 8.7, 25°C Bifidobacterium bifidum
2.7.7.12 0.031
-
 UDP-galactose pH 8.7, 25°C Bifidobacterium bifidum
2.7.7.12 0.14 0.16 alpha-D-glucose 1-phosphate pH 8.7, 25°C Bifidobacterium bifidum
2.7.7.12 0.3 0.5 alpha-D-galactose 1-phosphate pH 8.7, 25°C Bifidobacterium bifidum
2.7.7.12 6.66
-
 alpha-D-galactose 1-phosphate pH 8.7, 25°C Bifidobacterium bifidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.12 110000
-
 gel filtration Bifidobacterium bifidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate Bifidobacterium bifidum involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Bifidobacterium bifidum
-
 constitutive expression
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 213fold to homogeneity Bifidobacterium bifidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.12 5.53
-
 purified enzyme Bifidobacterium bifidum

Storage Stability

EC Number Storage Stability Organism
2.7.7.12 -20°C, in 0.01 M phosphate buffer, pH 7.5: 40% loss of activity within 2 weeks and 60% within a month, with 20% glycerol: 15% loss of activity within 2 months Bifidobacterium bifidum
2.7.7.12 4°C, in 0.01 M potassium phosphate buffer, pH 7.5, inactivation within 2 weeks, 2-mercaptoethanol, DTT and 20% glycerol slightly stabilize Bifidobacterium bifidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 CDP-glucose + alpha-D-galactose 1-phosphate no activity Bifidobacterium bifidum alpha-D-glucose 1-phosphate + CDP-galactose
-
 ?
2.7.7.12 additional information no activity with CDP-glucose Bifidobacterium bifidum ?
-
 ?
2.7.7.12 additional information no activity with ADP-glucose or GDP-glucose Bifidobacterium bifidum ?
-
 ?
2.7.7.12 TDP-glucose + alpha-D-galactose 1-phosphate reaction at 60% the rate of UDP-glucose Bifidobacterium bifidum alpha-D-glucose 1-phosphate + TDP-galactose
-
 r
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate highly specific for the substrates Bifidobacterium bifidum alpha-D-glucose 1-phosphate + UDP-galactose
-
 r
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate involved in conversion of galactose into alpha-D-glucose 1-phosphate, Leloir pathway Bifidobacterium bifidum alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.12 dimer 2 * 53000-55000, SDS-PAGE Bifidobacterium bifidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.12 37
-
-
 Bifidobacterium bifidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.12 additional information
-
 DTT does not prevent heat inactivation Bifidobacterium bifidum
2.7.7.12 40
-
 10 min, 20% loss of activity Bifidobacterium bifidum
2.7.7.12 50
-
 above, inactivation Bifidobacterium bifidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.12 7.5
-
-
 Bifidobacterium bifidum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.12 4.4 9 about half-maximal activity at pH 4.4 and pH 9.0 Bifidobacterium bifidum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.12 4.5
-
 and below, very unstable Bifidobacterium bifidum
2.7.7.12 6 9 stable Bifidobacterium bifidum