Literature summary extracted from

Wedekind, J.E.; Frey, P.A.; Rayment, I.
Crystallization and preliminary crystallographic analysis of galactose-1-phosphate uridylyltransferase from Escherichia coli (1994), Acta Crystallogr. Sect. D, 50, 329-331.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.7.12	3 to 4fold overexpression in strain BL21(DE3)	Escherichia coli

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.7.7.12	17.5-23 mg/ml recombinant protein, hanging drop vapour diffusion method in the presence of 4 mM substrate analog phenyl-UDP, 277 K, 0.1 M sodium succinate, pH 5.9, 0.25 M NaCl, 0.4 M Li2SO4, over 14.5% w/w polyethylene glycol 10000, 1 mM NaN3, 5-6 days, X-ray diffraction structure determination and analysis	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.12	Escherichia coli	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.12	recombinant from overexpressing strain BL21(DE3)	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.7.12	UDP-glucose + alpha-D-galactose 1-phosphate	-	Escherichia coli	alpha-D-glucose 1-phosphate + UDP-galactose	-	r

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Release 2023.1 (January 2023)