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Literature summary extracted from

  • Arabshahi, A.; Brody, R.S.; Smallwood, A.; Tsai, T.C.; Frey, P.A.
    Galactose-1-phosphate uridylyltransferase. Purification of the enzyme and stereochemical course of each step of the double-displacement mechanism (1986), Biochemistry, 25, 5583-5589.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.12 PMSF, EDTA and 2-mercaptoethanol stabilize during purification Escherichia coli
2.7.7.12 purified enzyme has tendency to undergo transition to a lower specific activity form Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate Escherichia coli
-
 alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose double-displacement mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.12 238
-
 purified enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.12 metal ions or/and substrates do not protect from oxidation or proteolysis during prolonged storage Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 UDP-alphaS-glucose + alpha-D-galactose 1-phosphate stereochemistry: enzyme accepts (Rp)-UDP-alphaS-glucose converting it to (Rp)-UDP-alphaS-galactose, the overall retention of configuration arises from inversion in each of the two steps Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose (Rp1)-UDP-alphaS-galactose r
2.7.7.12 UDP-alphaS-glucose + alpha-D-galactose 1-phosphate stereochemistry: enzyme accepts (Rp)-UDP-alphaS-glucose converting it to (Rp)-UDP-alphaS-galactose, the overall retention of configuration arises from inversion in each of the two steps Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose i.e. uridine 5'-(1-thiodiphosphate) galactose r
2.7.7.12 UDP-alphaS-glucose + alpha-D-galactose 1-phosphate i.e. uridine 5'-(1-thiodiphosphate) glucose Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose (Rp1)-UDP-alphaS-galactose r
2.7.7.12 UDP-alphaS-glucose + alpha-D-galactose 1-phosphate i.e. uridine 5'-(1-thiodiphosphate) glucose Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose i.e. uridine 5'-(1-thiodiphosphate) galactose r
2.7.7.12 UDP-alphaS-glucose + alpha-D-galactose 1-phosphate (Rp1)-UDP-alphaS-glucose Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose (Rp1)-UDP-alphaS-galactose r
2.7.7.12 UDP-alphaS-glucose + alpha-D-galactose 1-phosphate (Rp1)-UDP-alphaS-glucose Escherichia coli alpha-D-glucose 1-phosphate + UDP-alphaS-galactose i.e. uridine 5'-(1-thiodiphosphate) galactose r
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate
-
 Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
 r
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate stereochemistry: enzyme accepts (Rp)-UDP-alphaS-glucose converting it to (Rp)-UDP-alphaS-galactose, the overall retention of configuration arises from inversion in each of the two steps Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
 r
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate formation of a covalent uridylyl-enzyme intermediate, i.e. UMP-enzyme Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
 r