Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lobelle-Rich, P.A.; Reeves, R.E.
    Galactose-1-phosphate uridylyltransferase from Entamoeba histolytica (1982), Methods Enzymol., 90, 552-555.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.10 0.034
-
 UTP pH 7.0, 30°C Entamoeba histolytica
2.7.7.10 0.23
-
 alpha-D-glucose 1-phosphate pH 7.0, 30°C Entamoeba histolytica
2.7.7.10 0.415
-
 alpha-D-galactose 1-phosphate pH 7.0, 30°C Entamoeba histolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.10 Mg2+ activation Entamoeba histolytica
2.7.7.10 Mn2+ activation Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.10 40000
-
 gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.10 UTP + alpha-D-galactose 1-phosphate Entamoeba histolytica constitutive enzyme diphosphate + UDP-galactose
-
 r
2.7.7.10 UTP + alpha-D-galactose 1-phosphate Entamoeba histolytica K-9 / ATCC 30015 constitutive enzyme diphosphate + UDP-galactose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.10 Entamoeba histolytica
-
-
-
2.7.7.10 Entamoeba histolytica K-9 / ATCC 30015
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.10
-
 Entamoeba histolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.10 135
-
 purified enzyme Entamoeba histolytica

Storage Stability

EC Number Storage Stability Organism
2.7.7.10 -20°C, in 20 mM imidazole-HCl buffer, pH 7, 20% v/v glycerol, 30% loss of activity within 6 weeks Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.10 additional information no activity with ATP, ITP, CTP or GTP Entamoeba histolytica ?
-
 ?
2.7.7.10 additional information no activity with ATP, ITP, CTP or GTP Entamoeba histolytica K-9 / ATCC 30015 ?
-
 ?
2.7.7.10 UTP + alpha-D-galactose 1-phosphate
-
 Entamoeba histolytica diphosphate + UDP-galactose
-
 r
2.7.7.10 UTP + alpha-D-galactose 1-phosphate constitutive enzyme Entamoeba histolytica diphosphate + UDP-galactose
-
 r
2.7.7.10 UTP + alpha-D-galactose 1-phosphate
-
 Entamoeba histolytica K-9 / ATCC 30015 diphosphate + UDP-galactose
-
 r
2.7.7.10 UTP + alpha-D-galactose 1-phosphate constitutive enzyme Entamoeba histolytica K-9 / ATCC 30015 diphosphate + UDP-galactose
-
 r
2.7.7.10 UTP + alpha-D-glucose 1-phosphate reaction at 75% the rate of alpha-D-galactose 1-phosphate Entamoeba histolytica diphosphate + UDP-glucose
-
 r
2.7.7.10 UTP + alpha-D-glucose 1-phosphate reaction at 75% the rate of alpha-D-galactose 1-phosphate Entamoeba histolytica K-9 / ATCC 30015 diphosphate + UDP-glucose
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.10 30
-
 assay at Entamoeba histolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.10 7
-
 assay at Entamoeba histolytica