EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.7.1 | - |
Methanothermobacter thermautotrophicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.1 | H16A | slower turnover and higher KM-value for ATP then wild-type | Methanothermobacter thermautotrophicus |
2.7.7.1 | H19A | slower turnover but lower KM-value for ATP then wild-type | Methanothermobacter thermautotrophicus |
2.7.7.1 | R11A | slower turnover and higher KM-value for ATP then wild-type | Methanothermobacter thermautotrophicus |
2.7.7.1 | R11K | slower turnover and higher KM-value for ATP then wild-type | Methanothermobacter thermautotrophicus |
2.7.7.1 | R136A | slower turnover and higher KM-value for ATP then wild-type | Methanothermobacter thermautotrophicus |
2.7.7.1 | R136K | slower turnover and higher KM-value for ATP then wild-type | Methanothermobacter thermautotrophicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.1 | 0.08 | - |
NMN | 65°C, pH 7.5, wild-type | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.08 | - |
ATP | 65°C, pH 7.5, H19A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.09 | - |
NMN | 65°C, pH 7.5, H19A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.11 | - |
NMN | 65°C, pH 7.5, R136A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.13 | - |
NMN | 65°C, pH 7.5, R11K | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.14 | - |
NMN | 65°C, pH 7.5, R136K and R11A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.16 | - |
ATP | 65°C, pH 7.5, wild-type | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.2 | - |
NMN | 65°C, pH 7.5, H16A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.21 | - |
ATP | 65°C, pH 7.5, R11K | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.33 | - |
ATP | 65°C, pH 7.5, R11A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.46 | - |
ATP | 65°C, pH 7.5, R136K | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 1.1 | - |
ATP | 65°C, pH 7.5, H16A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 1.4 | - |
ATP | 65°C, pH 7.5, R136A | Methanothermobacter thermautotrophicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.1 | Methanothermobacter thermautotrophicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.1 | - |
Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.1 | ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Methanothermobacter thermautotrophicus | diphosphate + NAD+ | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.1 | 0.0000333 | - |
ATP | 65°C, pH 7.5, H19A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.000117 | - |
ATP | 65°C, pH 7.5, R136A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.000133 | - |
ATP | 65°C, pH 7.5, R11A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.000167 | - |
ATP | 65°C, pH 7.5, H16A | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.0008 | - |
ATP | 65°C, pH 7.5, R11K | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.00222 | - |
ATP | 65°C, pH 7.5, R136K | Methanothermobacter thermautotrophicus | |
2.7.7.1 | 0.00278 | - |
ATP | 65°C, pH 7.5, wild-type | Methanothermobacter thermautotrophicus |