Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Raffaelli, N.; Pisani, F.M.; Lorenzi, T.; Emanuelli, M.; Amici, A.; Ruggieri, S.; Magni, G.
    Nicotinamide-mononucleotide adenylyltransferase from Methanococcus jannaschii (2001), Methods Enzymol., 331, 292-298.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.1 SO42- at 0.1 M activation Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.1 expression in Escherichia coli BL21 Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.1 Co2+ most effective, 0.5 mM giving the same extent of activation like 10 mM Mg2+ Methanocaldococcus jannaschii
2.7.7.1 Cu2+
-
 Methanocaldococcus jannaschii
2.7.7.1 Fe3+
-
 Methanocaldococcus jannaschii
2.7.7.1 Mg2+
-
 Saccharolobus solfataricus
2.7.7.1 Mg2+
-
 Methanocaldococcus jannaschii
2.7.7.1 Mn2+
-
 Methanocaldococcus jannaschii
2.7.7.1 Ni2+ most effective, 0.5 mM giving the same extent of activation like 10 mM Mg2+ Methanocaldococcus jannaschii
2.7.7.1 Zn2+
-
 Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.1 18600
-
 x * 18600, SDS-PAGE Saccharolobus solfataricus
2.7.7.1 19600
-
 x * 19600, SDS-PAGE Methanocaldococcus jannaschii
2.7.7.1 66000
-
 gel filtration Saccharolobus solfataricus
2.7.7.1 72000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Methanocaldococcus jannaschii
-
-
-
2.7.7.1 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.1 8.8
-
-
 Saccharolobus solfataricus
2.7.7.1 187
-
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Saccharolobus solfataricus diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Methanocaldococcus jannaschii diphosphate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.1 ? x * 18600, SDS-PAGE Saccharolobus solfataricus
2.7.7.1 ? x * 19600, SDS-PAGE Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.1 70
-
 stable for 6 h Methanocaldococcus jannaschii
2.7.7.1 80
-
 half life: 3 h Methanocaldococcus jannaschii
2.7.7.1 90
-
 half life: 1 h Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.1 5.8 7.8
-
 Saccharolobus solfataricus