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Literature summary extracted from

  • Raffaelli, N.; Sorci, L.; Amici, A.; Emanuelli, M.; Mazzola, F.; Magni, G.
    Identification of a novel human nicotinamide mononucleotide adenylyltransferase (2002), Biochem. Biophys. Res. Commun., 297, 835-840.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.1 dithiothreitol stimulates the catalytic activity of NMNAT-2 up to 30% Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.1 expression in Escherichia coli, NMNAT-2 with a 6 x His-tag Homo sapiens
2.7.7.1 overexpression in HeLa and HEK293 cells as GFP-fusion protein Homo sapiens

General Stability

EC Number General Stability Organism
2.7.7.1 dithiothreitol stabalizes enzyme NMNAT-2 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.1 0.023
-
 ATP
-
 Homo sapiens
2.7.7.1 0.036
-
 ATP
-
 Homo sapiens
2.7.7.1 0.116
-
 nicotinic acid mononucleotide
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.1 divalent cations
-
 Homo sapiens
2.7.7.1 Mg2+ optimal concentration of NMNAT-2 is 0.3 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.1 33000
-
 gel filtration, recombinant NMNAT-2 with a 6 x His-tag Homo sapiens
2.7.7.1 34400
-
 calculated from nucleic acid sequence, NMNAT-2 Homo sapiens
2.7.7.1 36800
-
 1 * 36800, SDS-PAGE, recombinant NMNAT-2 with a 6 x His-tag Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide Homo sapiens
-
 diphosphate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1 recombinant enzyme NMNAT-2 Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.1 15
-
 NMNAT-2 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Homo sapiens diphosphate + NAD+
-
 ?
2.7.7.1 ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Homo sapiens diphosphate + NAD+
-
 r
2.7.7.1 ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Homo sapiens diphosphate + nicotinic acid-adenine dinucleotide
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.1 monomer 1 * 36800, SDS-PAGE, recombinant NMNAT-2 with a 6 x His-tag Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.1 6 9 recombinant NMNAT-2 Homo sapiens