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Literature summary extracted from

  • Sanemori, H.; Kawasaki, T.
    Purification and properties of thiamine pyrophosphokinase in Paracoccus denitrificans (1980), J. Biochem., 88, 223-230.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.2 Chloroethylthiamine weak Paracoccus denitrificans
2.7.6.2 iodoacetamide 3.3 mM, 23.3% inhibition, activity is recovered to approximately 90% on incubation with a 10fold excess of dithiothreitol Paracoccus denitrificans
2.7.6.2 oxythiamine weak Paracoccus denitrificans
2.7.6.2 PCMB 1 mM, 55.1% inhibition, activity is recovered to approximately 90% on incubation with a 10fold excess of dithiothreitol Paracoccus denitrificans
2.7.6.2 pyrithiamine
-
 Paracoccus denitrificans
2.7.6.2 thiamine diphosphate
-
 Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.2 0.01
-
 thiamine pH 9.0, 37°C Paracoccus denitrificans
2.7.6.2 0.38
-
 ATP pH 9.0, 37°C Paracoccus denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.2 Mg2+ required Paracoccus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.2 44000
-
 gel filtration Paracoccus denitrificans
2.7.6.2 96000
-
 aggregate of the functional dimer, equilibrium sedimentation Paracoccus denitrificans
2.7.6.2 230000
-
 2 * 230000, SDS-PAGE Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.2 Paracoccus denitrificans
-
-
-
2.7.6.2 Paracoccus denitrificans 12442
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.2
-
 Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.2 0.0235
-
-
 Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.2 ATP + thiamine
-
 Paracoccus denitrificans AMP + thiamine diphosphate
-
 ?
2.7.6.2 ATP + thiamine
-
 Paracoccus denitrificans 12442 AMP + thiamine diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.2 dimer 2 * 230000, SDS-PAGE Paracoccus denitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.2 40
-
-
 Paracoccus denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.2 9
-
-
 Paracoccus denitrificans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.6.2 0.019
-
 pyrithiamine
-
 Paracoccus denitrificans