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Literature summary extracted from

  • Krath, B.N.; Hove-Jensen, B.
    Class II recombinant phosphoribosyl diphosphate synthase from spinach. Phosphate independence and diphosphoryl donor specificity (2001), J. Biol. Chem., 276, 17851-17856.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate isoenzyme 3 is independent of phosphate Spinacia oleracea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expression of isoenzyme 3 in Escherichia coli Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 5-phospho-alpha-D-ribose 1-diphosphate competitive with respect to ATP and noncompetitive with respect to D-ribose 5-phosphate Spinacia oleracea
2.7.6.1 ADP
-
 Spinacia oleracea
2.7.6.1 AMP noncompetitive to both substrates Spinacia oleracea
2.7.6.1 GDP
-
 Spinacia oleracea
2.7.6.1 GTP substrate inhibition Spinacia oleracea
2.7.6.1 L-histidine no inhibition Spinacia oleracea
2.7.6.1 L-tryptophan no inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.11
-
 D-ribose 5-phosphate pH 7.6, 37ºC, isoenzyme 3 Spinacia oleracea
2.7.6.1 0.116
-
 CTP pH 7.6, 37ºC, isoenzyme 3 Spinacia oleracea
2.7.6.1 0.137
-
 UTP pH 7.6, 37ºC, isoenzyme 3 Spinacia oleracea
2.7.6.1 0.17
-
 ATP pH 7.6, 37ºC, isoenzyme 3 Spinacia oleracea
2.7.6.1 0.233
-
 dATP pH 7.6, 37ºC, isoenzyme 3 Spinacia oleracea
2.7.6.1 0.65
-
 GTP pH 7.6, 37ºC, isoenzyme 3 Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Cd2+ no activity observed Spinacia oleracea
2.7.6.1 Co2+ no activity observed Spinacia oleracea
2.7.6.1 Mg2+ requirement Spinacia oleracea
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Spinacia oleracea
2.7.6.1 Mn2+ can partially replace Mg2+ Spinacia oleracea
2.7.6.1 additional information no activity observed with Ca2+ Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Spinacia oleracea
-
 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 ammonium sulfate and polyethylene glycol precipitation, affinity chromatography and anion exchange chromatography, purification of isoenzyme 3 Spinacia oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction Spinacia oleracea
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate sequential kinetic mechanism Spinacia oleracea
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 13.1
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Spinacia oleracea AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 CTP + D-ribose 5-phosphate less effective than ATP Spinacia oleracea CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 dATP + D-ribose 5-phosphate equally effective as ATP Spinacia oleracea dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 GTP + D-ribose 5-phosphate less effective than ATP Spinacia oleracea GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 additional information 2-deoxyribose-5-phosphate is not substrate Spinacia oleracea ?
-
 ?
2.7.6.1 UTP + D-ribose 5-phosphate less effective than ATP Spinacia oleracea UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 37
-
 assay at Spinacia oleracea
2.7.6.1 40
-
-
 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 7.6
-
 activity increases up to pH 7.2 and decreases above pH 7.6 Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.6.1 0.45
-
 AMP pH 7.6, 37ºC, 5-phospho-alpha-D-ribose 1-diphosphate as substrate, isoenzyme 3 Spinacia oleracea
2.7.6.1 0.52
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.6, 37ºC, ATP as substrate, isoenzyme 3 Spinacia oleracea
2.7.6.1 0.68
-
 ADP pH 7.6, 37ºC, ATP as substrate, isoenzyme 3 Spinacia oleracea
2.7.6.1 0.82
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.6, 37ºC, 5-phospho-alpha-D-ribose 1-diphosphate as substrate, isoenzyme 3 Spinacia oleracea
2.7.6.1 1.22
-
 ADP pH 7.6, 37ºC, 5-phospho-alpha-D-ribose 1-diphosphate as substrate, isoenzyme 3 Spinacia oleracea
2.7.6.1 1.62
-
 AMP pH 7.6, 37ºC, ATP as substrate, isoenzyme 3 Spinacia oleracea