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Literature summary extracted from

  • Krath, B.N.; Hove-Jensen, B.
    Implications of secondary structure prediction and amino acid sequence comparison of class I and class II phosphoribosyl diphosphate synthases on catalysis, regulation, and quaternary structure (2001), Protein Sci., 10, 2317-2324.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate activity of the enzyme is independent of phosphate. Without effect up to 80 mM Spinacia oleracea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expression in Escherichia coli Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 5-phospho-alpha-D-ribose 1-diphosphate competitive with respect to ATP and noncompetitive with respect to D-ribose 5-phosphate Spinacia oleracea
2.7.6.1 ADP competitive inhibition to ATP Spinacia oleracea
2.7.6.1 AMP non competitive with respect to D-ribose 5-phosphate and ATP Spinacia oleracea
2.7.6.1 UTP substrate inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.048
-
 D-ribose 5-phosphate pH conditions not mentioned, 37ºC Spinacia oleracea
2.7.6.1 0.077
-
 ATP pH conditions not mentioned, 37ºC Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Co2+ no activity observed Spinacia oleracea
2.7.6.1 Mg2+ requirement Spinacia oleracea
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Spinacia oleracea
2.7.6.1 Mn2+ 25-30% of the activity with Mg2+ Spinacia oleracea
2.7.6.1 additional information no activity observed with Ca2+ Spinacia oleracea
2.7.6.1 additional information no activity observed with Cd2+, Cu2+, Fe2+ and Ni2+ Spinacia oleracea
2.7.6.1 Zn2+ no activity observed Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Spinacia oleracea
-
 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 streptomycin sulfate and ammonium sulfate precipitation, chromatography on Dyematrex Gel Blue B and DE52 Spinacia oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction Spinacia oleracea
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate sequential kinetic mechanism Spinacia oleracea
a

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 -20ºC, Tris/HCl buffer 50 mM, pH 7.6, 50% glycerol, stable Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Spinacia oleracea AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 CTP + D-ribose 5-phosphate
-
 Spinacia oleracea CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 dATP + D-ribose 5-phosphate
-
 Spinacia oleracea dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 GTP + D-ribose 5-phosphate
-
 Spinacia oleracea GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 UTP + D-ribose 5-phosphate
-
 Spinacia oleracea UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 37
-
 assay at Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 8.5
-
 37ºC, in Tris/ClH buffer Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.6.1 0.11
-
 ADP pH conditions not mentioned, 37ºC, ATP as substrate, Kis value Spinacia oleracea
2.7.6.1 0.16
-
 5-phospho-alpha-D-ribose 1-diphosphate pH conditions not mentioned, 37ºC, ATP as substrate, Kis value Spinacia oleracea
2.7.6.1 0.33
-
 5-phospho-alpha-D-ribose 1-diphosphate pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kis value Spinacia oleracea
2.7.6.1 0.38
-
 AMP pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kis value Spinacia oleracea
2.7.6.1 0.43
-
 AMP pH conditions not mentioned, 37ºC, ATP as substrate, Kis value Spinacia oleracea
2.7.6.1 2.2
-
 5-phospho-alpha-D-ribose 1-diphosphate pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kii value Spinacia oleracea
2.7.6.1 3.9
-
 AMP pH conditions not mentioned, 37ºC, D-ribose 5-phosphate as substrate, Kii value Spinacia oleracea
2.7.6.1 5.6
-
 AMP pH conditions not mentioned, 37ºC, ATP as substrate, Kii value Spinacia oleracea