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Literature summary extracted from

  • Kita, K.; Otsuki, T.; Ishizuka, T.; Tatibana, M.
    Rat liver phosphoribosyl pyrophosphate synthetase: existence of the purified enzyme as heterogeneous aggregates and identification of the catalytic subunit (1989), J. Biochem., 105, 736-741.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 additional information
-
 purified enzyme appears to exist as complex aggregates composed of heterogeneous components Rattus norvegicus
2.7.6.1 34000
-
 x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit Rattus norvegicus
2.7.6.1 38000
-
 x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit Rattus norvegicus
2.7.6.1 40000
-
 x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit Rattus norvegicus
2.7.6.1 1000000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Rattus norvegicus the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 polyethylene glycol precipitation, hydroxyapatite fractionation, gel filtration on Toyopearl HW-65F, chromatography on DEAE-Toyopearl 650S and gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 7.28
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 -80ºC, potassium phosphate buffer, pH 7.4, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.6.1 ? x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit Rattus norvegicus