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Literature summary extracted from

  • Fox, I.H.; Kelley, W.N.
    Human phosphoribosylpyrophosphate synthetase. Kinetic mechanism and end product inhibition (1972), J. Biol. Chem., 247, 2126-2131.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate
-
Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 2,3-diphosphoglycerate 30% inhibition at 1 mM; competitive to D-ribose 5-phosphate Homo sapiens
2.7.6.1 2-deoxy-D-ribose 5-phosphate 92% inhibition at 1 mM Homo sapiens
2.7.6.1 5-phospho-alpha-D-ribose 1-diphosphate competitive to ribose 5-phosphate Homo sapiens
2.7.6.1 adenosine 27% inhibition at 1 mM Homo sapiens
2.7.6.1 ADP 99% inhibition at 1 mM; competitive inhibition to ATP; end product inhibition; the most effective Homo sapiens
2.7.6.1 allopurinol ribonucleotides 23% inhibition at 1 mM Homo sapiens
2.7.6.1 AMP 69% inhibition at 1 mM Homo sapiens
2.7.6.1 CDP 44% inhibition at 1 mM Homo sapiens
2.7.6.1 CTP 40% inhibition at 1 mM Homo sapiens
2.7.6.1 D-fructose 1,6-diphosphate 20% inhibition at 1 mM Homo sapiens
2.7.6.1 D-Fructose 1-phosphate 19% inhibition at 1 mM Homo sapiens
2.7.6.1 D-ribose 1-phosphate 13% inhibition at 1 mM Homo sapiens
2.7.6.1 FAD 51% inhibition at 1 mM Homo sapiens
2.7.6.1 GDP
-
Homo sapiens
2.7.6.1 glyceraldehyde 3-phosphate 14% inhibition at 1 mM Homo sapiens
2.7.6.1 GMP 27% inhibition at 1 mM Homo sapiens
2.7.6.1 GTP 51% inhibition at 1 mM Homo sapiens
2.7.6.1 guanosine 20% inhibition at 1 mM Homo sapiens
2.7.6.1 IDP 47% inhibition at 1 mM Homo sapiens
2.7.6.1 IMP 21% inhibition at 1 mM Homo sapiens
2.7.6.1 ITP 36% inhibition at 1 mM Homo sapiens
2.7.6.1 additional information inhibition by pair of inhibitors tested is not greater than the sum of the inhibition produced by each alone Homo sapiens
2.7.6.1 NAD+ 100% inhibition at 1 mM Homo sapiens
2.7.6.1 NADH 72% inhibition at 1 mM Homo sapiens
2.7.6.1 NADPH 100% inhibition at 1 mM Homo sapiens
2.7.6.1 TDP 100% inhibition at 1 mM Homo sapiens
2.7.6.1 TTP 47% inhibition at 1 mM Homo sapiens
2.7.6.1 UDP 10%-16% inhibition at 1 mM Homo sapiens
2.7.6.1 UMP 10%-18% inhibition at 1 mM Homo sapiens
2.7.6.1 XDP 36% inhibition at 1 mM Homo sapiens
2.7.6.1 XTP 22% inhibition at 1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.0002
-
Mg2+ pH 7.4, 37ºC Homo sapiens
2.7.6.1 0.014
-
ATP pH 7.4, 37ºC Homo sapiens
2.7.6.1 0.014
-
ATP ATP in form of MgATP2- Homo sapiens
2.7.6.1 0.033
-
ribose 5-phosphate pH 7.4, 37ºC Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Homo sapiens the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 partial, 400fold purification Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction with ribose-5-phosphate binding first and 5-phosphoribose diphosphate being released last Homo sapiens
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 erythrocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 37
-
assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.6.1 0.1
-
5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 37ºC Homo sapiens
2.7.6.1 0.3
-
AMP pH 7.4, 37ºC, with D-ribose-5-phosphate Homo sapiens
2.7.6.1 0.4
-
AMP pH 7.4, 37ºC, with MgATP2- Homo sapiens