EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | additional information | at 50 and 100 mM, KCl, KHCO3 and CH3COOK have no activating effects | Rattus norvegicus | |
2.7.6.1 | phosphate | requirement | Rattus norvegicus | |
2.7.6.1 | phosphate | no activity at phosphate concentrations lower than 0.4 mM | Rattus norvegicus | |
2.7.6.1 | sulfate | can partially replace phosphate in activation | Rattus norvegicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.6.1 | expression in Escherichia coli | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | ADP | one of the most effective | Rattus norvegicus | |
2.7.6.1 | AMP | one of the most effective | Rattus norvegicus | |
2.7.6.1 | CTP | no inhibition | Rattus norvegicus | |
2.7.6.1 | GDP | one of the most effective | Rattus norvegicus | |
2.7.6.1 | GMP | no inhibition | Rattus norvegicus | |
2.7.6.1 | GTP | no inhibition | Rattus norvegicus | |
2.7.6.1 | UDP | no inhibition | Rattus norvegicus | |
2.7.6.1 | UMP | no inhibition | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 0.04 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC, isoenzyme PRSI | Rattus norvegicus | |
2.7.6.1 | 0.044 | - |
ATP | pH 7.4, 37ºC, isoenzyme PRSI | Rattus norvegicus | |
2.7.6.1 | 0.06 | - |
ATP | pH 7.4, 37ºC, isoenzyme PRSII | Rattus norvegicus | |
2.7.6.1 | 0.073 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC, isoenzyme PRSII | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 34000 | - |
x * 34000, SDS-PAGE, cloned and purified isoenzymes PRSI and PRSII | Rattus norvegicus |
2.7.6.1 | 550000 | - |
gel filtration, cloned and purified rPRSII | Rattus norvegicus |
2.7.6.1 | 700000 | 1200000 | gel filtration, cloned and purified rPRSI | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.6.1 | ATP + D-ribose 5-phosphate | Rattus norvegicus | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.6.1 | Rattus norvegicus | - |
rat | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.6.1 | polyethylene glycol, acid precipitation and chromatography on DEAE-5PW HPLC, purification of the two isoforms PRSI and PRSII | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.6.1 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 25.7 | - |
isoenzyme PRSI | Rattus norvegicus |
2.7.6.1 | 34.5 | - |
isoenzyme PRSII | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.6.1 | -80ºC, potassium phosphate 50 mM, pH 7.4 | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.6.1 | ATP + D-ribose 5-phosphate | - |
Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.7.6.1 | ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.6.1 | ? | x * 34000, SDS-PAGE, cloned and purified isoenzymes PRSI and PRSII | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 49 | - |
half-life of 0.5 min and 90 min for isoenzymes rPRSII and rPRSI, respectively | Rattus norvegicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.6.1 | Rattus norvegicus | isoenzyme rPRSII | 4.4 | 4.3 |
2.7.6.1 | Rattus norvegicus | isoenzyme rPRSI | - |
4.4 |