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Literature summary extracted from

  • Roth, D.G.; White, C.; Deuel, T.F.
    Ribosephosphate pyrophosphokinase (rat liver) (1978), Methods Enzymol., 51, 12-17.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 arsenate can partially replace phosphate in activation Rattus norvegicus
2.7.6.1 phosphate requirement Rattus norvegicus
2.7.6.1 sulfate can partially replace phosphate in activation Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.6.1 high enzyme concentration, albumin, 0.05 mg/ml, EDTA, 1 mM or DTT, 1 mM stabilizes during assay Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ADP competitive inhibition to ATP Rattus norvegicus
2.7.6.1 AMP noncompetitive to both substrates Rattus norvegicus
2.7.6.1 ATP free form, slight inhibition at 1 mM Rattus norvegicus
2.7.6.1 D-ribose 5-phosphate substrate inhibition above 1.5 mM Rattus norvegicus
2.7.6.1 dADP competitive to ATP Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.22
-
 ATP pH and temperature conditions not mentioned Rattus norvegicus
2.7.6.1 0.22
-
 ATP ATP in form of MgATP2- Rattus norvegicus
2.7.6.1 0.29
-
 5-phospho-D-ribose diphosphate pH and temperature conditions not mentioned Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 additional information
-
 purified enzyme is in an aggregated state Rattus norvegicus
2.7.6.1 40000
-
 x * 40000, SDS-PAGE with mercaptoethanol Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Rattus norvegicus the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 acetone powder, acid and streptomycin precipitation, heat treatment and agarose chromatography, 1500 to 3000fold purification Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 1.05
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 4°C, 50% loss of activity after 2 months. -20ºC, rapid loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for D-ribose 5-phosphate Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 dATP + D-ribose 5-phosphate
-
 Rattus norvegicus dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.6.1 ? x * 40000, SDS-PAGE with mercaptoethanol Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 8 8.8
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.6.1 0.15
-
 ADP pH and temperature conditions not mentioned Rattus norvegicus