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Literature summary extracted from

  • Zhang, Y.; Li, Y.; Wu, Y.; Yan, H.
    Structural and functional roles of tyrosine 78 of yeast guanylate kinase (1997), J. Biol. Chem., 272, 19343-19350.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.8 Y78F affinity for MgATP2- similar to wild type but affinity for GMP decreases by a factor of 12 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 GMP
-
 Saccharomyces cerevisiae
2.7.4.8 MgATP2-
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.091
-
 GMP pH 7.7, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.2
-
 MgATP2- pH 7.7, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.45
-
 MgATP2- pH 7.7, 25ºC, Y78F mutant Saccharomyces cerevisiae
2.7.4.8 1.8
-
 GMP pH 7.7, 25ºC, Y78F mutant Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 GMP + MgATP2-
-
 Saccharomyces cerevisiae MgADP- + GDP
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.8 0.035
-
 GMP pH 7.7, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.08
-
 MgATP2- pH 7.7, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.16
-
 MgATP2- pH 7.7, 25ºC, Y78F mutant Saccharomyces cerevisiae
2.7.4.8 0.63
-
 GMP pH 7.7, 25ºC, Y78F mutant Saccharomyces cerevisiae