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Literature summary extracted from

  • Li, Y.; Zhang, Y.; Yan, H.
    Kinetic and thermodynamic characterizations of yeast guanylate kinase (1996), J. Biol. Chem., 271, 28038-28044.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.8 expressed in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.8 S80A sluggish enzyme Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 GDP
-
 Saccharomyces cerevisiae
2.7.4.8 GMP non competitive with respect to MgATP2- because of the formation of an abortive complex guanylate kinase-MgATP2-GMP Saccharomyces cerevisiae
2.7.4.8 MgADP-
-
 Saccharomyces cerevisiae
2.7.4.8 MgATP2-
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.017
-
 MgADP- pH 7.5, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.091
-
 GMP pH 7.5, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.097
-
 GDP pH 7.5, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.2
-
 MgATP2- pH 7.5, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.23
-
 MgATP2- pH 7.5, 25ºC, S80A mutant Saccharomyces cerevisiae
2.7.4.8 0.38
-
 dGMP pH 7.5, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.5
-
 GMP pH 7.5, 25ºC, S80A mutant Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Mg2+
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + GMP
-
 Saccharomyces cerevisiae ADP + GDP
-
 ?
2.7.4.8 ATP + GMP
-
 Saccharomyces cerevisiae ADP + GDP
-
 r
2.7.4.8 GMP + MgATP2-
-
 Saccharomyces cerevisiae MgADP- + GDP
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.8 51
-
 dGMP pH 7.5, 25ºC Saccharomyces cerevisiae
2.7.4.8 90
-
 ATP pH 7.7, 25ºC, reverse reaction Saccharomyces cerevisiae
2.7.4.8 394
-
 GMP pH 7.7, 25ºC, forward reaction Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.8 0.035
-
 GMP pH 7.5, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.037
-
 MgADP- pH 7.5, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.08
-
 MgATP2- pH 7.5, 25ºC Saccharomyces cerevisiae
2.7.4.8 0.215
-
 GDP pH 7.5, 25ºC Saccharomyces cerevisiae