Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Le Floc'h, F.; Lafleuriel, J.
    Purification and properties of guanylate kinase of mitochondrias from tubers of Jerusalem artichoke (1990), Plant Physiol. Biochem., 28, 191-201.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 ATP free form, substrate inhibition, competitive toward MgATP2- Helianthus tuberosus
2.7.4.8 GDP GMP-phosphorylation Helianthus tuberosus
2.7.4.8 GTP GMP-phosphorylation Helianthus tuberosus
2.7.4.8 K+ weak Helianthus tuberosus
2.7.4.8 additional information guanylic nucleotides strongly inhibit, compete with GMP Helianthus tuberosus
2.7.4.8 phosphate weak Helianthus tuberosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.016
-
 GMP
-
 Helianthus tuberosus
2.7.4.8 0.42
-
 MgATP2-
-
 Helianthus tuberosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.8 mitochondrion
-
 Helianthus tuberosus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Co2+ can partially replace Mg2+ Helianthus tuberosus
2.7.4.8 Fe2+ activation, 90% as effective as Mg2+ Helianthus tuberosus
2.7.4.8 Mg2+ requirement Helianthus tuberosus
2.7.4.8 Mg2+ active substrate: MgATP2- Helianthus tuberosus
2.7.4.8 Mn2+ requirement Helianthus tuberosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.8 72000
-
 gel filtration Helianthus tuberosus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Helianthus tuberosus
-
 Jerusalem artichoke
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8 method that includes DEAE-Sephacel and Blue Sepharose CL 6B chromatography Helianthus tuberosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.8 tuber
-
 Helianthus tuberosus
-

Storage Stability

EC Number Storage Stability Organism
2.7.4.8 -25°C, stable Helianthus tuberosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + GMP
-
 Helianthus tuberosus ADP + GDP
-
 ?
2.7.4.8 dATP + dGMP
-
 Helianthus tuberosus dADP + dGDP
-
 ?
2.7.4.8 GMP + MgATP2-
-
 Helianthus tuberosus MgADP- + GDP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.8 7 8
-
 Helianthus tuberosus