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Literature summary extracted from

  • Oeschger, M.
    Guanylate kinase from Escherichia coli B (1978), Methods Enzymol., 51, 473-482.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.8 unstable in dilute solutions, below 0.005 mg/ml, bovine serum albumin or other suitable proteins protect, highly purified enzyme is stabilized by the presence of lactic dehydrogenase and pyruvate kinase Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.3
-
 dGMP pH 8.0, 37ºC, NH4+ as activator ion Escherichia coli
2.7.4.8 0.75
-
 Mn2+
-
 Escherichia coli
2.7.4.8 1
-
 Mg2+
-
 Escherichia coli
2.7.4.8 1.25
-
 Co2+
-
 Escherichia coli
2.7.4.8 10.8 347 K+
-
 Escherichia coli
2.7.4.8 39
-
 NH4+
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Co2+ activation Escherichia coli
2.7.4.8 K+ activation Escherichia coli
2.7.4.8 Mg2+
-
 Escherichia coli
2.7.4.8 Mn2+ requirement Escherichia coli
2.7.4.8 Mn2+ equally effective as Mg2+ Escherichia coli
2.7.4.8 Mn2+ 60% as effective as Mg2+ Escherichia coli
2.7.4.8 NH4+ activation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.8 88000
-
 equilibrium centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Escherichia coli
-
-
-
2.7.4.8 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8 method that includes Sephadex and two DEAE-cellulose chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.8 187
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.4.8 -20°C, stable at all stages of purification Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + dGMP
-
 Escherichia coli ADP + dGDP
-
 r
2.7.4.8 ATP + dGMP
-
 Escherichia coli B / ATCC 11303 ADP + dGDP
-
 r
2.7.4.8 ATP + GMP specificity Escherichia coli ADP + GDP
-
 ?
2.7.4.8 ATP + GMP specificity Escherichia coli B / ATCC 11303 ADP + GDP
-
 ?
2.7.4.8 dATP + dGMP
-
 Escherichia coli dADP + dGDP
-
 ?
2.7.4.8 dATP + dGMP
-
 Escherichia coli B / ATCC 11303 dADP + dGDP
-
 ?
2.7.4.8 dATP + GMP
-
 Escherichia coli dADP + GDP
-
 ?
2.7.4.8 dATP + GMP
-
 Escherichia coli B / ATCC 11303 dADP + GDP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.8 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.8 7.3 8.2 Tris-chloride buffer Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.8 6.5 9 70% of maximal activity at pH 6.5 and 9 Escherichia coli