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Literature summary extracted from

  • Nomura, T.; Fukui, T.; Ichikawa, A.
    Purification and characterization of nucleoside diphosphate kinase from spinach leaves (1991), Biochim. Biophys. Acta, 1077, 47-55.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.6 GDP at higher concentrations, substrate inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.6 0.091 0.1 GDP cosubstrate ATP, NDP-kinase I and II, 37°C, pH 7.4 Spinacia oleracea
2.7.4.6 0.89
-
ATP cosubstrate GDP, NDP-kinase II, 37°C, pH 7.4 Spinacia oleracea
2.7.4.6 2
-
ATP NDP-kinase I, 37°C, pH 7.4 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 cytosol
-
Spinacia oleracea 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 additional information
-
amino acid composition Spinacia oleracea
2.7.4.6 16000
-
6 * 16000, isozyme I, SDS-PAGE Spinacia oleracea
2.7.4.6 18000
-
6 * 18000, SDS-PAGE, isozyme II Spinacia oleracea
2.7.4.6 92000
-
isozyme I, gel filtration Spinacia oleracea
2.7.4.6 110000
-
isozyme II, gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 2 isozymes: NDP-kinase I and II Spinacia oleracea

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.6 ATP + nucleoside diphosphate = ADP + nucleoside triphosphate the catalytic mechanism involves a phosphoenzyme intermediate Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.6 leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.6 1250
-
NDP-kinase I, 37°C, pH 7.4 Spinacia oleracea
2.7.4.6 2160
-
NDP-kinase II, 37°C, pH 7.4 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.7.4.6 -20°C, 0.05 M 2-(N-morpholino)ethanesulfonic acid, pH 5.5, 1 mM EDTA, 0.2 mM PMSF, 0.1 M NaCl, 5 mM 2-mercaptoethanol, 0.1% Lubrol, several months Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 gamma-S-ATP + GDP poor substrate Spinacia oleracea ADP + gamma-S-GTP
-
?
2.7.4.6 gamma-S-GTP + GDP poor substrate Spinacia oleracea GDP + gamma-S-GTP
-
?
2.7.4.6 GTP + ADP
-
Spinacia oleracea GDP + ATP
-
r
2.7.4.6 NTP + nucleoside diphosphate
-
Spinacia oleracea NDP + NTP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 hexamer 6 * 16000, isozyme I, SDS-PAGE Spinacia oleracea
2.7.4.6 hexamer 6 * 18000, SDS-PAGE, isozyme II Spinacia oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.6 30
-
assay at Spinacia oleracea
2.7.4.6 37
-
assay at Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.6 7.5
-
-
Spinacia oleracea