BRENDA - Enzyme Database

Purification and characterization of adenylate kinase from rat liver

Criss, W.E.; Pradhan, T.K.; Methods Enzymol. 51, 459-467 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.3
2-oxoglutarate
activation
Rattus norvegicus
2.7.4.3
alanine
activation
Rattus norvegicus
2.7.4.3
CDP
activation
Rattus norvegicus
2.7.4.3
cis-aconitate
activation
Rattus norvegicus
2.7.4.3
citrate
activation
Rattus norvegicus
2.7.4.3
dithiothreitol
not
Rattus norvegicus
2.7.4.3
fumarate
activation
Rattus norvegicus
2.7.4.3
GDP
activation
Rattus norvegicus
2.7.4.3
isocitrate
activation
Rattus norvegicus
2.7.4.3
L-histidine
activation
Rattus norvegicus
2.7.4.3
malate
activation
Rattus norvegicus
2.7.4.3
additional information
no activation by GTP, CTP, UTP, TTP, 2'-dGTP, 2'-dCTP, 2'-dATP, GMP, CMP, UMP, TMP, IMP, 2'-dAMP or 3',5'-cAMP; no activation by succinate or oxaloacetate
Rattus norvegicus
2.7.4.3
Prostaglandins
activation
Rattus norvegicus
2.7.4.3
TDP
activation
Rattus norvegicus
2.7.4.3
threonine
activation
Rattus norvegicus
2.7.4.3
UDP
activation
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.7.4.3
repeated freeze-thawing inactivates
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.3
oleic acid
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.3
1.9
-
AMP
37°C, pH 7.0
Rattus norvegicus
2.7.4.3
7
-
ATP
37°C, pH 7.0
Rattus norvegicus
2.7.4.3
16.8
-
ADP
37°C, pH 7.0
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.3
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.3
Mg2+
-
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.3
11000
-
2 * 13000 + 1 * 11000, SDS-PAGE
Rattus norvegicus
2.7.4.3
13000
-
2 * 13000 + 1 * 11000, SDS-PAGE
Rattus norvegicus
2.7.4.3
46000
49000
analytical ultracentrifugation
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.3
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.3
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.3
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.3
1000
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.7.4.3
4°C, 5 mM sodium phosphate, pH 7, 1 mM 2-mercaptoethanol, 1 month
Rattus norvegicus
2.7.4.3
frozen, up to 6 months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.3
ADP + ADP
-
642602
Rattus norvegicus
ATP + AMP
-
-
-
r
2.7.4.3
ATP + AMP
no substrates are ATP/dGMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are 3',5'-cAMP, dAMP, 2'-AMP, 3'-AMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are ATP/IMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are ATP/UMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are ATP/TMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are ATP/GMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
dATP + AMP
-
642602
Rattus norvegicus
dADP + ADP
-
-
-
?
2.7.4.3
dGTP + AMP
-
642602
Rattus norvegicus
dGDP + ADP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.4.3
trimer
2 * 13000 + 1 * 11000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.4.3
37
-
assay at
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.3
2-oxoglutarate
activation
Rattus norvegicus
2.7.4.3
alanine
activation
Rattus norvegicus
2.7.4.3
CDP
activation
Rattus norvegicus
2.7.4.3
cis-aconitate
activation
Rattus norvegicus
2.7.4.3
citrate
activation
Rattus norvegicus
2.7.4.3
dithiothreitol
not
Rattus norvegicus
2.7.4.3
fumarate
activation
Rattus norvegicus
2.7.4.3
GDP
activation
Rattus norvegicus
2.7.4.3
isocitrate
activation
Rattus norvegicus
2.7.4.3
L-histidine
activation
Rattus norvegicus
2.7.4.3
malate
activation
Rattus norvegicus
2.7.4.3
additional information
no activation by GTP, CTP, UTP, TTP, 2'-dGTP, 2'-dCTP, 2'-dATP, GMP, CMP, UMP, TMP, IMP, 2'-dAMP or 3',5'-cAMP; no activation by succinate or oxaloacetate
Rattus norvegicus
2.7.4.3
Prostaglandins
activation
Rattus norvegicus
2.7.4.3
TDP
activation
Rattus norvegicus
2.7.4.3
threonine
activation
Rattus norvegicus
2.7.4.3
UDP
activation
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.4.3
repeated freeze-thawing inactivates
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.3
oleic acid
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.3
1.9
-
AMP
37°C, pH 7.0
Rattus norvegicus
2.7.4.3
7
-
ATP
37°C, pH 7.0
Rattus norvegicus
2.7.4.3
16.8
-
ADP
37°C, pH 7.0
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.3
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.3
Mg2+
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.3
11000
-
2 * 13000 + 1 * 11000, SDS-PAGE
Rattus norvegicus
2.7.4.3
13000
-
2 * 13000 + 1 * 11000, SDS-PAGE
Rattus norvegicus
2.7.4.3
46000
49000
analytical ultracentrifugation
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.3
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.3
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.3
1000
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.4.3
4°C, 5 mM sodium phosphate, pH 7, 1 mM 2-mercaptoethanol, 1 month
Rattus norvegicus
2.7.4.3
frozen, up to 6 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.3
ADP + ADP
-
642602
Rattus norvegicus
ATP + AMP
-
-
-
r
2.7.4.3
ATP + AMP
no substrates are ATP/dGMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are 3',5'-cAMP, dAMP, 2'-AMP, 3'-AMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are ATP/IMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are ATP/UMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are ATP/TMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
ATP + AMP
no substrates are ATP/GMP
642602
Rattus norvegicus
ADP + ADP
-
642602
Rattus norvegicus
r
2.7.4.3
dATP + AMP
-
642602
Rattus norvegicus
dADP + ADP
-
-
-
?
2.7.4.3
dGTP + AMP
-
642602
Rattus norvegicus
dGDP + ADP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.4.3
trimer
2 * 13000 + 1 * 11000, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.4.3
37
-
assay at
Rattus norvegicus