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Literature summary extracted from

  • Storey, K.B.
    Purification and properties of squid mantle adenylate kinase. Role of NADH in control of the enzyme (1976), J. Biol. Chem., 251, 7810-7815.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.3 (NH4)2SO4 inhibitory above 30 mM, activates below Doryteuthis pealeii
2.7.4.3 2-mercaptoethanol activation Doryteuthis pealeii
2.7.4.3 citrate not Doryteuthis pealeii
2.7.4.3 dithiothreitol activation Doryteuthis pealeii
2.7.4.3 KCl activation Doryteuthis pealeii
2.7.4.3 L-cysteine activation Doryteuthis pealeii
2.7.4.3 NH4Cl activation Doryteuthis pealeii

General Stability

EC Number General Stability Organism
2.7.4.3 NADH or MgATP2- or ATP plus AMP protect against proteolysis by pronase or trypsin and against heat denaturation Doryteuthis pealeii
2.7.4.3 unstable in dilute solutions and in the absence of SH-compounds Doryteuthis pealeii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 (NH4)2SO4 above 30 mM, activates below Doryteuthis pealeii
2.7.4.3 arginine phosphate weak Doryteuthis pealeii
2.7.4.3 LiCl
-
 Doryteuthis pealeii
2.7.4.3 additional information not inhibitory: citrate Doryteuthis pealeii
2.7.4.3 Na2SO4
-
 Doryteuthis pealeii
2.7.4.3 NAD+
-
 Doryteuthis pealeii
2.7.4.3 NADH
-
 Doryteuthis pealeii
2.7.4.3 NH4Cl
-
 Doryteuthis pealeii
2.7.4.3 phosphoenolpyruvate
-
 Doryteuthis pealeii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 0.13
-
 ATP
-
 Doryteuthis pealeii
2.7.4.3 0.15
-
 2 ADP cosubstrate AMP Doryteuthis pealeii
2.7.4.3 0.15
-
 2 ADP ADP in form of MgADP- Doryteuthis pealeii
2.7.4.3 0.65 0.7 ADP
-
 Doryteuthis pealeii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 cytosol
-
 Doryteuthis pealeii 5829
-
2.7.4.3 mitochondrion
-
 Doryteuthis pealeii 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Mg2+
-
 Doryteuthis pealeii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 27000
-
 gel filtration Doryteuthis pealeii

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Doryteuthis pealeii
-
 squid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3 no isozymes Doryteuthis pealeii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.3 muscle mantle muscle Doryteuthis pealeii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.3 2700
-
-
 Doryteuthis pealeii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 2 ADP
-
 Doryteuthis pealeii ATP + AMP
-
 ?
2.7.4.3 ADP + ADP
-
 Doryteuthis pealeii ATP + AMP
-
 r
2.7.4.3 ATP + AMP
-
 Doryteuthis pealeii ADP + ADP
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.3 39
-
 t1/2: 11 min Doryteuthis pealeii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.3 7.4
-
 AMP + ATP Doryteuthis pealeii
2.7.4.3 8.2
-
 ADP + ADP Doryteuthis pealeii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.3 4.5
-
 unstable at Doryteuthis pealeii