BRENDA - Enzyme Database

Purification and properties of squid mantle adenylate kinase. Role of NADH in control of the enzyme

Storey, K.B.; J. Biol. Chem. 251, 7810-7815 (1976)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.3
(NH4)2SO4
inhibitory above 30 mM, activates below
Doryteuthis pealeii
2.7.4.3
2-mercaptoethanol
activation
Doryteuthis pealeii
2.7.4.3
citrate
not
Doryteuthis pealeii
2.7.4.3
dithiothreitol
activation
Doryteuthis pealeii
2.7.4.3
KCl
activation
Doryteuthis pealeii
2.7.4.3
L-cysteine
activation
Doryteuthis pealeii
2.7.4.3
NH4Cl
activation
Doryteuthis pealeii
General Stability
EC Number
General Stability
Organism
2.7.4.3
NADH or MgATP2- or ATP plus AMP protect against proteolysis by pronase or trypsin and against heat denaturation
Doryteuthis pealeii
2.7.4.3
unstable in dilute solutions and in the absence of SH-compounds
Doryteuthis pealeii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.3
(NH4)2SO4
above 30 mM, activates below
Doryteuthis pealeii
2.7.4.3
arginine phosphate
weak
Doryteuthis pealeii
2.7.4.3
LiCl
-
Doryteuthis pealeii
2.7.4.3
additional information
not inhibitory: citrate
Doryteuthis pealeii
2.7.4.3
Na2SO4
-
Doryteuthis pealeii
2.7.4.3
NAD+
-
Doryteuthis pealeii
2.7.4.3
NADH
-
Doryteuthis pealeii
2.7.4.3
NH4Cl
-
Doryteuthis pealeii
2.7.4.3
phosphoenolpyruvate
-
Doryteuthis pealeii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.3
0.13
-
ATP
-
Doryteuthis pealeii
2.7.4.3
0.15
-
MgADP-
cosubstrate AMP
Doryteuthis pealeii
2.7.4.3
0.65
0.7
ADP
-
Doryteuthis pealeii
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.3
cytosol
-
Doryteuthis pealeii
5829
-
2.7.4.3
mitochondrion
-
Doryteuthis pealeii
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.3
Mg2+
-
Doryteuthis pealeii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.3
27000
-
gel filtration
Doryteuthis pealeii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.3
Doryteuthis pealeii
-
squid
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.3
no isozymes
Doryteuthis pealeii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.3
muscle
mantle muscle
Doryteuthis pealeii
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.3
2700
-
-
Doryteuthis pealeii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.3
ADP + ADP
-
642600
Doryteuthis pealeii
ATP + AMP
-
-
-
r
2.7.4.3
ATP + AMP
-
642600
Doryteuthis pealeii
ADP + ADP
-
642600
Doryteuthis pealeii
r
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.4.3
39
-
t1/2: 11 min
Doryteuthis pealeii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.3
7.4
-
AMP + ATP
Doryteuthis pealeii
2.7.4.3
8.2
-
ADP + ADP
Doryteuthis pealeii
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.7.4.3
4.5
-
unstable at
Doryteuthis pealeii
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.4.3
(NH4)2SO4
inhibitory above 30 mM, activates below
Doryteuthis pealeii
2.7.4.3
2-mercaptoethanol
activation
Doryteuthis pealeii
2.7.4.3
citrate
not
Doryteuthis pealeii
2.7.4.3
dithiothreitol
activation
Doryteuthis pealeii
2.7.4.3
KCl
activation
Doryteuthis pealeii
2.7.4.3
L-cysteine
activation
Doryteuthis pealeii
2.7.4.3
NH4Cl
activation
Doryteuthis pealeii
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.4.3
NADH or MgATP2- or ATP plus AMP protect against proteolysis by pronase or trypsin and against heat denaturation
Doryteuthis pealeii
2.7.4.3
unstable in dilute solutions and in the absence of SH-compounds
Doryteuthis pealeii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.4.3
(NH4)2SO4
above 30 mM, activates below
Doryteuthis pealeii
2.7.4.3
arginine phosphate
weak
Doryteuthis pealeii
2.7.4.3
LiCl
-
Doryteuthis pealeii
2.7.4.3
additional information
not inhibitory: citrate
Doryteuthis pealeii
2.7.4.3
Na2SO4
-
Doryteuthis pealeii
2.7.4.3
NAD+
-
Doryteuthis pealeii
2.7.4.3
NADH
-
Doryteuthis pealeii
2.7.4.3
NH4Cl
-
Doryteuthis pealeii
2.7.4.3
phosphoenolpyruvate
-
Doryteuthis pealeii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.3
0.13
-
ATP
-
Doryteuthis pealeii
2.7.4.3
0.15
-
MgADP-
cosubstrate AMP
Doryteuthis pealeii
2.7.4.3
0.65
0.7
ADP
-
Doryteuthis pealeii
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.3
cytosol
-
Doryteuthis pealeii
5829
-
2.7.4.3
mitochondrion
-
Doryteuthis pealeii
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.3
Mg2+
-
Doryteuthis pealeii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.3
27000
-
gel filtration
Doryteuthis pealeii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.3
no isozymes
Doryteuthis pealeii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.4.3
muscle
mantle muscle
Doryteuthis pealeii
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.3
2700
-
-
Doryteuthis pealeii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.3
ADP + ADP
-
642600
Doryteuthis pealeii
ATP + AMP
-
-
-
r
2.7.4.3
ATP + AMP
-
642600
Doryteuthis pealeii
ADP + ADP
-
642600
Doryteuthis pealeii
r
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.4.3
39
-
t1/2: 11 min
Doryteuthis pealeii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.3
7.4
-
AMP + ATP
Doryteuthis pealeii
2.7.4.3
8.2
-
ADP + ADP
Doryteuthis pealeii
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.7.4.3
4.5
-
unstable at
Doryteuthis pealeii