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Literature summary extracted from

  • Lacher, K.; Schäfer, G.
    Archaebacterial adenylate kinase from the thermoacidophile Sulfolobus acidocaldarius: purification, characterization, and partial sequence (1993), Arch. Biochem. Biophys., 302, 391-397.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 P1,P5-diadenosine 5'-pentaphosphate weak Sulfolobus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 0.5 0.6 AMP cosubstrate ATP, 70°C Sulfolobus acidocaldarius
2.7.4.3 0.65 0.7 ADP 70°C Sulfolobus acidocaldarius
2.7.4.3 1.1
-
 ATP 90°C Sulfolobus acidocaldarius
2.7.4.3 1.3 1.37 AMP 90°C Sulfolobus acidocaldarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 cytosol
-
 Sulfolobus acidocaldarius 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Ca2+ in decreasing order of efficiency, substrates ADP + ADP: Mg2+, Mn2+, Zn2+, Ca2+ Sulfolobus acidocaldarius
2.7.4.3 Ca2+ in decreasing order of efficiency, substrates AMP + ATP: Mg2+, Mn2+, Ca2+, Zn2+ Sulfolobus acidocaldarius
2.7.4.3 Ca2+ residual activity even in the presence of EDTA Sulfolobus acidocaldarius
2.7.4.3 Mg2+ requirement Sulfolobus acidocaldarius
2.7.4.3 Mg2+ in decreasing order of efficiency, substrates ADP + ADP: Mg2+, Mn2+, Zn2+, Ca2+ Sulfolobus acidocaldarius
2.7.4.3 Mg2+ in decreasing order of efficiency, substrates AMP + ATP: Mg2+, Mn2+, Ca2+, Zn2+ Sulfolobus acidocaldarius
2.7.4.3 Mg2+ residual activity even in the presence of EDTA Sulfolobus acidocaldarius
2.7.4.3 Mn2+ in decreasing order of efficiency, substrates ADP + ADP: Mg2+, Mn2+, Zn2+, Ca2+ Sulfolobus acidocaldarius
2.7.4.3 Mn2+ in decreasing order of efficiency, substrates AMP + ATP: Mg2+, Mn2+, Ca2+, Zn2+ Sulfolobus acidocaldarius
2.7.4.3 Mn2+ residual activity even in the presence of EDTA Sulfolobus acidocaldarius
2.7.4.3 Zn2+ in decreasing order of efficiency, substrates ADP + ADP: Mg2+, Mn2+, Zn2+, Ca2+ Sulfolobus acidocaldarius
2.7.4.3 Zn2+ in decreasing order of efficiency, substrates AMP + ATP: Mg2+, Mn2+, Ca2+, Zn2+ Sulfolobus acidocaldarius
2.7.4.3 Zn2+ residual activity even in the presence of EDTA Sulfolobus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 23500
-
 2 * 23500, SDS-PAGE Sulfolobus acidocaldarius
2.7.4.3 52000
-
 gel filtration Sulfolobus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Sulfolobus acidocaldarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3
-
 Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.3 12.15
-
 45°C Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP + ADP
-
 Sulfolobus acidocaldarius ATP + AMP
-
 r
2.7.4.3 ATP + AMP no substrates are GTP/GMP, TTP/TMP Sulfolobus acidocaldarius ADP + ADP
-
 r
2.7.4.3 ATP + AMP highly specific for AMP Sulfolobus acidocaldarius ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/UMP Sulfolobus acidocaldarius ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/TMP Sulfolobus acidocaldarius ADP + ADP
-
 r
2.7.4.3 ATP + AMP no substrates are ATP/GMP Sulfolobus acidocaldarius ADP + ADP
-
 r
2.7.4.3 CTP + AMP reaction at 68% the rate of ATP Sulfolobus acidocaldarius ADP + CDP
-
 ?
2.7.4.3 dATP + AMP reaction at 80% the rate of ATP Sulfolobus acidocaldarius dADP + ADP
-
 ?
2.7.4.3 GTP + AMP reaction at 71% the rate of AMP Sulfolobus acidocaldarius ADP + GDP
-
 ?
2.7.4.3 ITP + AMP reaction at 58% the rate of ATP Sulfolobus acidocaldarius IDP + ADP
-
 ?
2.7.4.3 UTP + AMP reaction at 53% the rate of AMP Sulfolobus acidocaldarius ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.3 dimer 2 * 23500, SDS-PAGE Sulfolobus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.3 90
-
-
 Sulfolobus acidocaldarius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.4.3 70 95 about half-maximal activity at 70°C and about 75% of maximal activity at 95°C Sulfolobus acidocaldarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.3 55
-
 24 h, stable at pH 6 Sulfolobus acidocaldarius
2.7.4.3 65 70 12 h, 16% loss of activity, 24 h, 23% loss of activity, variation of ionic strength or addition of substrates does not stabilize Sulfolobus acidocaldarius
2.7.4.3 85
-
 3 h, inactivation Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.3 5.3
-
 AMP + ATP, 70°C Sulfolobus acidocaldarius
2.7.4.3 6
-
 AMP + ATP, 70°C Sulfolobus acidocaldarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.3 3.5 7 about half-maximal activity at pH 3.5 and about 70% of maximal activity at pH 7, ADP + ADP, 70°C Sulfolobus acidocaldarius
2.7.4.3 4 7.5 about half-maximal activity at pH 4 and about 70% of maximal activity at pH 7.5, AMP + ATP, 70°C Sulfolobus acidocaldarius

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.3 1 3 2 h, up to 15% loss of activity Sulfolobus acidocaldarius