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Literature summary extracted from

  • Tomasselli, A.G.; Noda, L.H.
    Mitochondrial ATP:AMP phosphotransferase from beef heart: purification and properties (1980), Eur. J. Biochem., 103, 481-491.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.3 NaCl activation, 0.5-0.8 M Bos taurus

General Stability

EC Number General Stability Organism
2.7.4.3 stable to repeated freeze-thaw cycles, at 5-10 mg/ml Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 AMP substrate inhibition Bos taurus
2.7.4.3 Butanedione
-
 Bos taurus
2.7.4.3 P1,P5-diadenosine 5'-pentaphosphate strong Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.3 0.114 0.13 AMP
-
 Bos taurus
2.7.4.3 0.34 0.35 ADP cosubstrate ATP Bos taurus
2.7.4.3 0.65 0.7 ADP
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 cytosol cytosolic and mitochondrial kinases are distinct isozymes Bos taurus 5829
-
2.7.4.3 mitochondrion cytosolic and mitochondrial kinases are distinct isozymes Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Ba2+ not Bos taurus
2.7.4.3 Ca2+ in decreasing order of efficiency, but not for reaction of ADP + ADP: Mg2+, Co2+, Ca2+, Mn2+, Ni2+ Bos taurus
2.7.4.3 Co2+ requirement Bos taurus
2.7.4.3 Mg2+ requirement Bos taurus
2.7.4.3 Mg2+ in decreasing order of efficiency, but no reaction of ADP + ADP: Mg2+, Co2+, Ca2+, Mn2+, Ni2+ Bos taurus
2.7.4.3 Mn2+ in decreasing order of efficiency, but not for reaction of ADP + ADP: Mg2+, Co2+, Ca2+, Mn2+, Ni2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 additional information
-
 comparison of amino acid composition of different sources Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.3 ATP + AMP = 2 ADP mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.3 heart
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.7.4.3 0°C, at least 2 days Bos taurus
2.7.4.3 deep frozen, 10-13% loss of activity within 6 months Bos taurus
2.7.4.3 room temperature, at least 2 days Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP + ADP
-
 Bos taurus ATP + AMP
-
 r
2.7.4.3 ATP + AMP best substrates Bos taurus ADP + ADP
-
 r
2.7.4.3 ATP + AMP highly specific for AMP Bos taurus ADP + ADP
-
 r
2.7.4.3 ATP + AMP less specific for ATP Bos taurus ADP + ADP
-
 r
2.7.4.3 ATP + dAMP reaction at 30% the rate of AMP Bos taurus ADP + dADP
-
 ?
2.7.4.3 CTP + AMP reaction at 12% the rate of ATP Bos taurus ADP + CDP
-
 ?
2.7.4.3 dATP + AMP at the same rate as ATP Bos taurus dADP + ADP
-
 ?
2.7.4.3 GTP + AMP reaction at 5% the rate of AMP Bos taurus ADP + GDP
-
 ?
2.7.4.3 ITP + AMP reaction at 10% the rate of ATP Bos taurus IDP + ADP
-
 ?
2.7.4.3 UTP + AMP reaction at 20% the rate of AMP Bos taurus ADP + UDP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.3 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.3 5.8
-
-
 Bos taurus
2.7.4.3 8
-
 ADP + ADP Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.3 5 11 1 h stable, 0.1-0.2 mg/ml, at 0°C Bos taurus
2.7.4.3 8
-
 at least 2 days Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.3 Bos taurus mitochondrial enzyme, isoelectric focusing
-
 9.3
2.7.4.3 Bos taurus cytosolic enzyme
-
 10.1