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Literature summary extracted from

  • Hall, S.W.; Kühn, H.
    Purification and properties of guanylate kinase from bovine retinas and rod outer segments (1986), Eur. J. Biochem., 161, 551-556.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 Ca2+ in the presence of Mg2+ Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.013
-
 GMP
-
 Bos taurus
2.7.4.8 0.43
-
 ATP
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 cytosol
-
 Bos taurus 5829
-
2.7.4.8 soluble
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Ca2+ no activator Bos taurus
2.7.4.8 Co2+ can partially replace Mg2+ Bos taurus
2.7.4.8 Mg2+
-
 Bos taurus
2.7.4.8 Mn2+ requirement Bos taurus
2.7.4.8 Zn2+ can partially replace Mg2+ Bos taurus
2.7.4.8 Zn2+ activation Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.8 20000
-
 gel filtration Bos taurus
2.7.4.8 23000
-
 1 * 23000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.8 ATP + GMP Bos taurus first step in 'cGMP-cycle' toward re-synthesis of cGMP ADP + GDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Bos taurus
-
-
-
2.7.4.8 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.3 ATP + AMP = 2 ADP mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.8 retina
-
 Bos taurus
-
2.7.4.8 retinal rod outer segment Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.3 additional information
-
-
 Bos taurus
2.7.4.8 323
-
 rod outer segment Bos taurus
2.7.4.8 354
-
 retina Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ATP + AMP
-
 Bos taurus ADP + ADP
-
 ?
2.7.4.8 ATP + dGMP phosphorylation at 48% the rate of GMP Bos taurus ADP + dGDP
-
 r
2.7.4.8 ATP + GDP
-
 Bos taurus ADP + GTP
-
 ?
2.7.4.8 ATP + GMP best substrates Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP AMP is no acceptor substrate Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP dAMP is no acceptor substrate Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP no donor substrates are GTP, CTP, UTP Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP CMP, UMP are no acceptor substrates Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP first step in 'cGMP-cycle' toward re-synthesis of cGMP Bos taurus ADP + GDP
-
 ?
2.7.4.8 dATP + dGMP phosphorylation at 22% the rate of ATP Bos taurus dADP + dGDP
-
 ?
2.7.4.8 dATP + GMP phosphorylation at 81% the rate of ATP Bos taurus dADP + GDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.8 monomer 1 * 23000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.8 30
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.8 130
-
 GTP calculated as GDP produced Bos taurus