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Literature summary extracted from

  • Nishino, H.
    Biogenesis of cocarboxylase in Escherichia coli. Partial purification and some properties of thiamine monophosphate kinase (1972), J. Biochem., 72, 1093-1100.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.16 glycerol stabilizes during purification Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.16 ADP product inhibition Escherichia coli
2.7.4.16 AMP
-
 Escherichia coli
2.7.4.16 Cs+ antagonizes stimulation by K+ Escherichia coli
2.7.4.16 diphosphate
-
 Escherichia coli
2.7.4.16 EDTA
-
 Escherichia coli
2.7.4.16 Li+ antagonizes stimulation by K+ Escherichia coli
2.7.4.16 additional information no inhibition by phosphate, KCN, iodoacetic acid, arsenate or arsenite Escherichia coli
2.7.4.16 N-ethylmaleimide
-
 Escherichia coli
2.7.4.16 Na+ antagonizes stimulation by K+ Escherichia coli
2.7.4.16 NaF weak Escherichia coli
2.7.4.16 oxythiamine
-
 Escherichia coli
2.7.4.16 PCMB 2-mercaptoethanol reverses Escherichia coli
2.7.4.16 pyrithiamine
-
 Escherichia coli
2.7.4.16 Pyrithiamine phosphate
-
 Escherichia coli
2.7.4.16 thiamine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.16 0.0011
-
 thiamine phosphate pH 7.5, 37°C Escherichia coli
2.7.4.16 0.27
-
 ATP pH 7.5, 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.16 soluble
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.16 Co2+ requirement, about 95% as effective as Mg2+ Escherichia coli
2.7.4.16 Fe2+ activation, about 10% as effective as Mg2+ Escherichia coli
2.7.4.16 K+ activation Escherichia coli
2.7.4.16 Mg2+ requirement Escherichia coli
2.7.4.16 Mn2+ activation, about 30% as effective as Mg2+ Escherichia coli
2.7.4.16 additional information no activation by Ba2+, Ca2+, Cu2+, Cs+, Li+, Na+ Escherichia coli
2.7.4.16 NH4+ activation Escherichia coli
2.7.4.16 Rb+ activation Escherichia coli
2.7.4.16 Zn2+ activation, about 30% as effective as Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.16 ATP + thiamine phosphate Escherichia coli
-
 ADP + thiamine diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.16 Escherichia coli
-
 K12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.16 partial Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.16 0.000197
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.4.16 -20°C, 3 days, t1/2: 10 days Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.16 ADP + thiamine phosphate poor substrate Escherichia coli ?
-
 ?
2.7.4.16 ATP + thiamine phosphate
-
 Escherichia coli ADP + thiamine diphosphate
-
 ?
2.7.4.16 ATP + thiamine phosphate no substrates: thiamine, GTP, CTP, UTP, AMP Escherichia coli ADP + thiamine diphosphate
-
 ?
2.7.4.16 ITP + thiamine phosphate poor substrate Escherichia coli ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.16 8
-
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.16 0.36
-
 pyrithiamine pH 7.5, 37°C Escherichia coli