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Literature summary extracted from

  • Mikoulinskaia, G.V.; Gubanov, S.I.; Zimin, A.A.; Kolesnikov, I.V.; Feofanov, S.A.; Miroshnikov, A.I.
    Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5 (2003), Protein Expr. Purif., 27, 195-201.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.13 29000
-
 1 * 29000, SDS-PAGE Tequintavirus T5
2.7.4.13 29140
-
 analytical equilibrium ultracentrifugation Tequintavirus T5

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.13 ATP + deoxynucleoside phosphate Tequintavirus T5 enzyme induced during viral infection ADP + deoxynucleoside diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.13 no activity in Escherichia coli
-
-
-
2.7.4.13 Tequintavirus T5
-
 from infected Escherichia coli cells
-
2.7.4.13 Tequintavirus T5
-
 mutant T5amH128a
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.13 1190fold to homogeneity Tequintavirus T5

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.13 166.8
-
 purified enzyme, substrate dTMP Tequintavirus T5
2.7.4.13 232
-
 purified enzyme, substrate dAMP Tequintavirus T5

Storage Stability

EC Number Storage Stability Organism
2.7.4.13 -20°C, purified enzyme, 15% glycerol, only loss of a small part of activity after 6 months Tequintavirus T5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.13 ATP + dAMP dAMP is the best acceptor substrate Tequintavirus T5 ADP + dADP
-
 r
2.7.4.13 ATP + dCMP phosphorylated at 36% the rate of dAMP Tequintavirus T5 ADP + dCDP
-
 r
2.7.4.13 ATP + deoxynucleoside phosphate enzyme induced during viral infection Tequintavirus T5 ADP + deoxynucleoside diphosphate
-
 r
2.7.4.13 ATP + dGMP phosphorylated at 65% the rate of dAMP Tequintavirus T5 ADP + dGDP
-
 r
2.7.4.13 ATP + dTMP phosphorylated at 70% the rate of dAMP Tequintavirus T5 ADP + dTDP
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.13 monomer 1 * 29000, SDS-PAGE Tequintavirus T5

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.13 37
-
 60 min, stable Tequintavirus T5
2.7.4.13 42
-
 10% loss of activity after 30 min Tequintavirus T5
2.7.4.13 47
-
 50% loss of activity after 30 min Tequintavirus T5
2.7.4.13 52
-
 rapid inactivation, t1/2: 5 min Tequintavirus T5

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.13 7 7.5
-
 Tequintavirus T5

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.13 additional information
-
 active over a broad range Tequintavirus T5

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.13 Tequintavirus T5 determined under partially denaturing condiions
-
 4.2