Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Albrecht, G.J.
    Purification and properties of nucleoside triphosphate-adenosine monophosphate transphosphorylase from beef heart mitochondria (1970), Biochemistry, 9, 2462-2470.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.10 ammonium sulfate or substrates enhance stability Bos taurus
2.7.4.10 freeze-thawing, dilutions, or low ionic strength decreases activity rapidly Bos taurus
2.7.4.10 stability increases during purification Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.10 ADP reverse reaction, kinetics Bos taurus
2.7.4.10 AMP free form, Mg2+ reverses Bos taurus
2.7.4.10 ATP
-
 Bos taurus
2.7.4.10 GDP reverse reaction, kinetics Bos taurus
2.7.4.10 GTP kinetics Bos taurus
2.7.4.10 Mg2+ weak, above 4 mM, activates below Bos taurus
2.7.4.10 additional information no inhibition by EDTA, GSH, cysteamine or high phosphate concentrations Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.10 0.0012
-
 GDP pH 8.0, with ADP Bos taurus
2.7.4.10 0.033
-
 AMP
-
 Bos taurus
2.7.4.10 0.056
-
 GTP pH 8.0, with AMP Bos taurus
2.7.4.10 0.29
-
 ADP pH 8.0, with GDP Bos taurus
2.7.4.10 0.63
-
 ITP pH 8.0, with AMP Bos taurus
2.7.4.10 1
-
 ATP pH 8.0, with of AMP Bos taurus
2.7.4.10 7.4
-
 UTP pH 8.0, with of AMP Bos taurus
2.7.4.10 9.1
-
 CTP pH 8.0, with of AMP Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.10 mitochondrion matrix Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.10 Ca2+ activation, 50% as effective as Mg2+ Bos taurus
2.7.4.10 Mg2+ activation, 4 mM, as good as Mn2+, slightly inhibitory above 4 mM Bos taurus
2.7.4.10 Mn2+ activation, as good as Mg2+ Bos taurus
2.7.4.10 additional information no activation by EDTA, cysteamine, GSH or high phosphate concentrations Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.10 52000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.10 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.10
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.10 nucleoside triphosphate + AMP = nucleoside diphosphate + ADP mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.10 heart
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.10 135
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.4.10 0°C, partially purified preparation, several weeks Bos taurus
2.7.4.10 room temperature, 1 week Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.10 ATP + AMP poor substrate, highly specific for AMP, phosphorylation at about 5% the rate of ITP Bos taurus 2 ADP
-
 r
2.7.4.10 CTP + AMP highly specific for AMP, phosphorylation at about 11% the rate of ITP Bos taurus CDP + ADP
-
 r
2.7.4.10 GTP + AMP highly specific for AMP and ADP, in reverse reaction Bos taurus GDP + ADP via nucleotide-enzyme complex, no phosphorylated enzyme intermediate r
2.7.4.10 GTP + AMP phosphorylation at about 90% the rate of ITP Bos taurus GDP + ADP via nucleotide-enzyme complex, no phosphorylated enzyme intermediate r
2.7.4.10 ITP + AMP best substrate Bos taurus IDP + ADP via nucleotide-enzyme complex, no phosphorylated enzyme intermediate r
2.7.4.10 ITP + AMP highly specific for AMP and ADP in reverse reaction Bos taurus IDP + ADP via nucleotide-enzyme complex, no phosphorylated enzyme intermediate r
2.7.4.10 ITP + AMP no acceptor substrates are 3'-GMP Bos taurus IDP + ADP via nucleotide-enzyme complex, no phosphorylated enzyme intermediate r
2.7.4.10 ITP + AMP not: IMP, CMP, UMP Bos taurus IDP + ADP via nucleotide-enzyme complex, no phosphorylated enzyme intermediate r
2.7.4.10 UTP + AMP highly specific for AMP, phosphorylation at about 19% the rate of ITP Bos taurus UDP + ADP
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.10 additional information
-
 35% ammonium sulfate, AMP or other substrates enhance thermal stability Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.10 8.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.10 7 9.7 about half-maximal activity at pH 7 and 9.7 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.10 0.73
-
 ATP pH 8.5, competitive with AMP Bos taurus
2.7.4.10 0.74
-
 ATP pH 8.5, competitive with GTP Bos taurus
2.7.4.10 0.77
-
 GDP pH 8.5 Bos taurus
2.7.4.10 0.8
-
 GTP pH 8.5 Bos taurus
2.7.4.10 0.9
-
 ADP pH 8.5 Bos taurus
2.7.4.10 1.6
-
 AMP pH 8.5 Bos taurus