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Literature summary extracted from

  • Saier, M.H.; Schmidt, M.R.; Lin, P.
    Phosphoryl exchange reaction catalyzed by enzyme I of the bacterial phosphoenolpyruvate: sugar phosphotransferase system. Kinetic characterization (1980), J. Biol. Chem., 255, 8579-8584.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.3.9 Co2+ activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 Mg2+ activation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 Mn2+ activation Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.9 2-Bromoacetyl-1-phosphonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 2-phosphoglycerate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 3-phosphoglycerate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 acetylphosphonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 Bromopyruvate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 Methylacetylphosphonate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 oxalate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.9 0.3
-
 phosphoenolpyruvate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.9 Co2+ can substitute for Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.9 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.3.9 phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine in a second reaction step phosphate is transferred to the N-1 position of the imidazole ring of the histidine containing-protein (i.e. HPr), the phospho group of the intermediate phospho-enzyme I can also be transferred to pyruvate or 2-oxobutyrate Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.9 alpha-ketobutyrate + phosphorylated histidine-containing protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphoenolbutyrate + histidine-containing protein
-
 ?
2.7.3.9 beta-hydroxypyruvate + phosphorylated histidine-containing protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium ? + histidine-containing protein
-
 ?
2.7.3.9 phosphoenolpyruvate + histidine-containing protein in a second reaction step phosphate is transferred to the N-1 position of the imidazole ring of the histidine containing-protein (i.e. HPr), the phospho group of the intermediate phospho-enzyme I can also be transferred to pyruvate or 2-oxobutyrate Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + phosphorylated histidine-containing protein
-
 r
2.7.3.9 pyruvate + phosphorylated histidine-containing protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphoenolpyruvate + histidine-containing protein
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.9 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.3.9 47.5
-
 stable up to, phosphoenolpyruvate stabilizes, Mg2+ destabilizes Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.9 8.2
-
 in presence of Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium