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Literature summary extracted from

  • Kukuruzinska, M.A.; Weigel, N.; Waygood, E.B.
    Enzyme I from Salmonella typhimurium (1982), Methods Enzymol., 90, 431-436.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.3.9
-
 Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

EC Number General Stability Organism
2.7.3.9 labile in purified state, sensitive to sulfhydryl reagents Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.9 Co2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 sulfhydryl reagents
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 Zn2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.9 0.0054
-
 histidine-containing protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 0.195 0.2 phosphoenolpyruvate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.9 Co2+ Km: 0.05 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 Mg2+ Km: 0.53 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 Mn2+ Km: 0.05 mM Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.9 70000
-
 monomer, gel filtration at 4-6°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 130000 150000 gel filtration at room temperature Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.9 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.9
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.3.9 phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine bi-bi ping-pong mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a
2.7.3.9 phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine in a first reaction step phosphate is transferred to the N-3 position of the imidazole ring of histidine Salmonella enterica subsp. enterica serovar Typhimurium
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.9 8929
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.7.3.9 -18°C, 0.05 M potassium phosphate buffer, pH 6.5, 0.2 mM dithioerythritol, 1 mM EDTA, several months Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.9 phosphoenolpyruvate + histidine-containing protein in a first reaction step phosphate is transferred to the N-3 position of the imidazole ring of histidine Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + phosphorylated histidine-containing protein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.9 dimer 2 * 57000-70000, gel filtration under denaturing conditions Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 dimer 2 * 57000-70000, sedimentation equilibrium centrifugation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.3.9 dimer 2 * 57000-70000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.9 7.2
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.3.9 0.7
-
 Co2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium