Literature summary extracted from

Hoving, H.; Koning, J.H.; Robillard, G.T.
Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: role of divalent metals in the dimerization and phosphorylation of enzyme I (1982), Biochemistry, 21, 3128-3136.

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.7.3.9	Mg2+	necessary for dimerization, only the dimeric enzyme can be phosphorylated	Escherichia coli
2.7.3.9	Mn2+	can substitute for Mg2+	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.3.9	Escherichia coli	-	-	-

Storage Stability

EC Number	Storage Stability	Organism
2.7.3.9	-20°C, 25 mM sodium phosphate buffer, pH 7.2, 1 mM NaN3, dithiothreitol, stable for months, but instable in Tris buffer	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.3.9	phosphoenolpyruvate + histidine-containing protein	-	Escherichia coli	pyruvate + phosphorylated histidine-containing protein	-	r
2.7.3.9	pyruvate + phosphorylated histidine-containing protein	-	Escherichia coli	phosphoenolpyruvate + histidine-containing protein	-	r

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Release 2023.1 (January 2023)