Literature summary extracted from

Vadeboncoeur, C.; Proulx, M.; Trahan, L.
Purification of proteins similar to HPr and enzyme I from the oral bacterium Streptococcus salivarius. Biochemical and immunochemical properties (1983), Can. J. Microbiol., 29, 1694-1705.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.3.9	0.026	0.031	histidine-containing protein	37°C, pH 7.0	Streptococcus salivarius
2.7.3.9	0.47	0.48	phosphoenolpyruvate	37°C, pH 7.0	Streptococcus salivarius

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.7.3.9	135000	-	size exclusion chromatography	Streptococcus salivarius

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.3.9	Streptococcus salivarius	-	immunologically distinct from Escherichia coli or other Streptococci	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.3.9	-	Streptococcus salivarius

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
2.7.3.9	phosphoenolpyruvate + protein histidine = pyruvate + protein Npi-phospho-L-histidine	ping-pong mechanism	Streptococcus salivarius	a

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7.3.9	530	-	37°C, pH 7.0	Streptococcus salivarius

Storage Stability

EC Number	Storage Stability	Organism
2.7.3.9	-20°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1 M KCl, 20% glycerol, no significant loss of activity in several months	Streptococcus salivarius

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.3.9	phosphoenolpyruvate + histidine-containing protein	-	Streptococcus salivarius	pyruvate + phosphorylated histidine-containing protein	-	?

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Release 2023.1 (January 2023)