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Literature summary extracted from

  • Gaffney, T.J.; Rosenberg, H.; Ennor, A.H.
    The purification and properties of adenosine triphosphate-lombricine phosphotransferase (1964), Biochem. J., 90, 170-176.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.3.5 freeze-drying of preparations in non-volatile buffers causes almost 40% loss of activity. Freezing and thawing at pH 8.6 in N-ethylmorpholine-hydrochloric acid buffer produces complete inactivation Megascolides cameroni

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.5 NEM 0.3 mM, complete inhibition Megascolides cameroni
2.7.3.5 o-Iodosobenzoate 0.3 mM, complete inhibition Megascolides cameroni
2.7.3.5 p-hydroxymercuribenzoate 0.0001 mM, complete inhibition Megascolides cameroni
2.7.3.5 Phenyl iodoacetate 1 mM, complete inhibition Megascolides cameroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.5 7.4
-
phospholombricine pH 8.6, 30°C Megascolides cameroni
2.7.3.5 9.1
-
D-lombricine pH 8.6, 30°C Megascolides cameroni
2.7.3.5 11.7
-
L-lombricine pH 8.6, 30°C Megascolides cameroni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.5 Ca2+ 5 mM, slight activation in both directions Megascolides cameroni
2.7.3.5 Co2+ 5 mM, slight activation in both directions Megascolides cameroni
2.7.3.5 Mg2+ 5 mM, activation in both directions Megascolides cameroni
2.7.3.5 Mn2+ 5 mM, activation in both directions Megascolides cameroni
2.7.3.5 additional information not activated by Ni2+, Cu2+, Fe2+, Fe3+, Al3+, Sn2+, Ba2+, Cd2+, Zn2+, Be2+ Megascolides cameroni

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.5 Megascolides cameroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.5
-
Megascolides cameroni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.5 muscle
-
Megascolides cameroni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.5 31
-
D-lombricine Megascolides cameroni
2.7.3.5 49
-
L-lombricine Megascolides cameroni

Storage Stability

EC Number Storage Stability Organism
2.7.3.5 2°C, pH 7.5, 50% v/v glycerol, several months stable Megascolides cameroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.5 ATP + D-lombricine
-
Megascolides cameroni ADP + phospholombricine
-
r
2.7.3.5 ATP + L-lombricine
-
Megascolides cameroni ADP + phospholombricine
-
r
2.7.3.5 ATP + lombricine i.e. guanidinoethylphospho-O-serine Megascolides cameroni ADP + phospholombricine
-
r
2.7.3.5 dADP + phospholombricine at 16% of the activity with ADP Megascolides cameroni dATP + lombricine
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.5 7.2
-
synthesis of ATP Megascolides cameroni
2.7.3.5 8.6
-
phosphorylation of lombricine Megascolides cameroni

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.3.5 6.5 8 pH 6.5: about 85% of maximal activity, pH 8.0: about 50% of maximal activity, synthesis of ATP Megascolides cameroni
2.7.3.5 7.5 9.5 pH 7.5: about 65% of maximal activity, pH 9.5: about 45% of maximal activity, phosphorylation of lombricine Megascolides cameroni