BRENDA - Enzyme Database

Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites

Pereira, C.A.; Alonso, G.D.; Paveto, M.C.; Iribarren, A.; Cabanas, M.L.; Torres, H.N.; Flawia, M.M.; J. Biol. Chem. 275, 1495-1501 (2000)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.7.3.3
medicine
arginine kinase is a possible chemotherapy target for Chagas‘ disease
Trypanosoma cruzi
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.3.3
expression in Escherichia coli
Trypanosoma cruzi
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.3
agmatine
5fold higher concentration than L-Arg, 20% inhibition
Trypanosoma cruzi
2.7.3.3
canavanine
5fold higher concentration than L-Arg, 50% inhibition
Trypanosoma cruzi
2.7.3.3
Ethylguanidine
5fold higher concentration than L-Arg, 22% inhibition
Trypanosoma cruzi
2.7.3.3
His
5fold higher concentration than L-Arg, 50% inhibition
Trypanosoma cruzi
2.7.3.3
L-Asp
5fold higher concentration than L-Arg, 25% inhibition
Trypanosoma cruzi
2.7.3.3
L-canavanine
-
Trypanosoma cruzi
2.7.3.3
L-Glu
5fold higher concentration than L-Arg, 31% inhibition
Trypanosoma cruzi
2.7.3.3
L-homoarginine
5fold higher concentration than L-Arg, 33% inhibition
Trypanosoma cruzi
2.7.3.3
L-Lys
5fold higher concentration than L-Arg, 25% inhibition
Trypanosoma cruzi
2.7.3.3
L-nitroarginine
5fold higher concentration than L-Arg, 28% inhibition
Trypanosoma cruzi
2.7.3.3
N-methyl-L-Arg
5fold higher concentration than L-Arg, 28% inhibition
Trypanosoma cruzi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.3
-
ATP
30°C, pH 7.3
Trypanosoma cruzi
2.7.3.3
0.3
-
L-Arg
30°C, pH 7.3
Trypanosoma cruzi
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Ca2+
a divalent cation such as Mg2+, Mn2+ or Ca2+ is required
Trypanosoma cruzi
2.7.3.3
Mg2+
a divalent cation such as Mg2+, Mn2+ or Ca2+ is required
Trypanosoma cruzi
2.7.3.3
Mn2+
a divalent cation such as Mg2+, Mn2+ or Ca2+ is required
Trypanosoma cruzi
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40201
-
x * 40201, calculation from nucleotide sequence
Trypanosoma cruzi
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Trypanosoma cruzi
O96507
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642482
Trypanosoma cruzi
ADP + Nomega-phospho-L-Arg
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.3.3
?
x * 40201, calculation from nucleotide sequence
Trypanosoma cruzi
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
8.2
-
-
Trypanosoma cruzi
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.3.3
6
-
L-canavanine
pH 7.3, 30°C
Trypanosoma cruzi
2.7.3.3
7
-
L-homoarginine
pH 7.3, 30°C
Trypanosoma cruzi
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.3.3
Trypanosoma cruzi
theoretical value calculated from amino acid sequence
-
7
Application (protein specific)
EC Number
Application
Commentary
Organism
2.7.3.3
medicine
arginine kinase is a possible chemotherapy target for Chagas‘ disease
Trypanosoma cruzi
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
expression in Escherichia coli
Trypanosoma cruzi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.3
agmatine
5fold higher concentration than L-Arg, 20% inhibition
Trypanosoma cruzi
2.7.3.3
canavanine
5fold higher concentration than L-Arg, 50% inhibition
Trypanosoma cruzi
2.7.3.3
Ethylguanidine
5fold higher concentration than L-Arg, 22% inhibition
Trypanosoma cruzi
2.7.3.3
His
5fold higher concentration than L-Arg, 50% inhibition
Trypanosoma cruzi
2.7.3.3
L-Asp
5fold higher concentration than L-Arg, 25% inhibition
Trypanosoma cruzi
2.7.3.3
L-canavanine
-
Trypanosoma cruzi
2.7.3.3
L-Glu
5fold higher concentration than L-Arg, 31% inhibition
Trypanosoma cruzi
2.7.3.3
L-homoarginine
5fold higher concentration than L-Arg, 33% inhibition
Trypanosoma cruzi
2.7.3.3
L-Lys
5fold higher concentration than L-Arg, 25% inhibition
Trypanosoma cruzi
2.7.3.3
L-nitroarginine
5fold higher concentration than L-Arg, 28% inhibition
Trypanosoma cruzi
2.7.3.3
N-methyl-L-Arg
5fold higher concentration than L-Arg, 28% inhibition
Trypanosoma cruzi
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.3.3
6
-
L-canavanine
pH 7.3, 30°C
Trypanosoma cruzi
2.7.3.3
7
-
L-homoarginine
pH 7.3, 30°C
Trypanosoma cruzi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.3
-
ATP
30°C, pH 7.3
Trypanosoma cruzi
2.7.3.3
0.3
-
L-Arg
30°C, pH 7.3
Trypanosoma cruzi
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Ca2+
a divalent cation such as Mg2+, Mn2+ or Ca2+ is required
Trypanosoma cruzi
2.7.3.3
Mg2+
a divalent cation such as Mg2+, Mn2+ or Ca2+ is required
Trypanosoma cruzi
2.7.3.3
Mn2+
a divalent cation such as Mg2+, Mn2+ or Ca2+ is required
Trypanosoma cruzi
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40201
-
x * 40201, calculation from nucleotide sequence
Trypanosoma cruzi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642482
Trypanosoma cruzi
ADP + Nomega-phospho-L-Arg
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.3.3
?
x * 40201, calculation from nucleotide sequence
Trypanosoma cruzi
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
8.2
-
-
Trypanosoma cruzi
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.3.3
Trypanosoma cruzi
theoretical value calculated from amino acid sequence
-
7