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Literature summary extracted from

  • Pereira, C.A.; Alonso, G.D.; Paveto, M.C.; Iribarren, A.; Cabanas, M.L.; Torres, H.N.; Flawia, M.M.
    Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites (2000), J. Biol. Chem., 275, 1495-1501.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.3.3 medicine arginine kinase is a possible chemotherapy target for Chagas‘ disease Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3 expression in Escherichia coli Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.3 agmatine 5fold higher concentration than L-Arg, 20% inhibition Trypanosoma cruzi
2.7.3.3 canavanine 5fold higher concentration than L-Arg, 50% inhibition Trypanosoma cruzi
2.7.3.3 Ethylguanidine 5fold higher concentration than L-Arg, 22% inhibition Trypanosoma cruzi
2.7.3.3 His 5fold higher concentration than L-Arg, 50% inhibition Trypanosoma cruzi
2.7.3.3 L-Asp 5fold higher concentration than L-Arg, 25% inhibition Trypanosoma cruzi
2.7.3.3 L-canavanine
-
 Trypanosoma cruzi
2.7.3.3 L-Glu 5fold higher concentration than L-Arg, 31% inhibition Trypanosoma cruzi
2.7.3.3 L-homoarginine 5fold higher concentration than L-Arg, 33% inhibition Trypanosoma cruzi
2.7.3.3 L-Lys 5fold higher concentration than L-Arg, 25% inhibition Trypanosoma cruzi
2.7.3.3 L-nitroarginine 5fold higher concentration than L-Arg, 28% inhibition Trypanosoma cruzi
2.7.3.3 N-methyl-L-Arg 5fold higher concentration than L-Arg, 28% inhibition Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 0.3
-
 ATP 30°C, pH 7.3 Trypanosoma cruzi
2.7.3.3 0.3
-
 L-Arg 30°C, pH 7.3 Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.3 Ca2+ a divalent cation such as Mg2+, Mn2+ or Ca2+ is required Trypanosoma cruzi
2.7.3.3 Mg2+ a divalent cation such as Mg2+, Mn2+ or Ca2+ is required Trypanosoma cruzi
2.7.3.3 Mn2+ a divalent cation such as Mg2+, Mn2+ or Ca2+ is required Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 40201
-
 x * 40201, calculation from nucleotide sequence Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Trypanosoma cruzi O96507
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + L-Arg
-
 Trypanosoma cruzi ADP + Nomega-phospho-L-Arg
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.3 ? x * 40201, calculation from nucleotide sequence Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.3 8.2
-
-
 Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.3.3 6
-
 L-canavanine pH 7.3, 30°C Trypanosoma cruzi
2.7.3.3 7
-
 L-homoarginine pH 7.3, 30°C Trypanosoma cruzi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.3.3 Trypanosoma cruzi theoretical value calculated from amino acid sequence
-
 7