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Literature summary extracted from

  • Robin, Y.; Klotz, C.; van Thoai, N.
    Unspecific arginine kinase of molecular weight 150000 (1971), Eur. J. Biochem., 21, 170-178.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 0.8
-
 L-Arg pH 8.5, 25°C Sabella pavonina
2.7.3.3 1.3
-
 D-Arg pH 8.5, 25°C Sabella pavonina
2.7.3.3 1.4
-
 ATP pH 8.5, 25°C Sabella pavonina
2.7.3.3 1.4
-
 ATP ATP in form of MgATP2- Sabella pavonina
2.7.3.3 15
-
 octopine pH 8.5, 25°C Sabella pavonina
2.7.3.3 15
-
 5-guanidinopentanoic acid pH 8.5, 25°C Sabella pavonina
2.7.3.3 18
-
 L-argininic acid pH 8.5, 25°C Sabella pavonina
2.7.3.3 30
-
 N-acetyl-L-Arg
-
 Sabella pavonina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 150000
-
 sedimentation equilibrium centrifugation Sabella pavonina

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Sabella pavonina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3
-
 Sabella pavonina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.3 body wall muscle
-
 Sabella pavonina
-

Storage Stability

EC Number Storage Stability Organism
2.7.3.3 refrigerated, 80% saturated ammonium sulfate, 20 mM L-Arg, 100 mM 2-mercaptoethanol, pH 7.0. 50% loss of activity in 8-10 days Sabella pavonina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + 4-guanidinebutanoic acid 8% of the activity with L-Arg Sabella pavonina ADP + N-phospho-4-guanidinobutanoic acid
-
 ?
2.7.3.3 ATP + 5-guanidinopentanoic acid 10% of the activity with L-Arg Sabella pavonina ADP + N-phospho-5-guanidinopentanoic acid
-
 ?
2.7.3.3 ATP + D-Arg D-Arg is as active as L-Arg Sabella pavonina ADP + Nomega-phospho-D-Arg
-
 ?
2.7.3.3 ATP + L-Arg strictly specific for ATP Sabella pavonina ADP + Nomega-phospho-L-Arg
-
 r
2.7.3.3 ATP + L-arginine
-
 Sabella pavonina ADP + Nomega-phospho-L-arginine
-
 r
2.7.3.3 ATP + L-argininic acid 45% of the activity with L-Arg Sabella pavonina ADP + Nomega-phospho-L-argininic acid
-
 ?
2.7.3.3 ATP + L-canavanine 7% of the activity with L-Arg Sabella pavonina ADP + L-phosphocanavanine
-
 r
2.7.3.3 ATP + L-homoarginine 25% of the activity with L-Arg Sabella pavonina ADP + Nomega-phospho-L-homoarginine
-
 ?
2.7.3.3 ATP + N-acetyl-L-Arg 13% of the activity with L-Arg Sabella pavonina ADP + Nomega-phospho-N-alpha-acetyl-L-Arg
-
 ?
2.7.3.3 ATP + octopine 30% of the activity with L-Arg Sabella pavonina ADP + N-phospho-D-octopine
-
 ?
2.7.3.3 additional information strictly specific for ATP Sabella pavonina ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.3 6.2 6.3 synthesis of ATP Sabella pavonina
2.7.3.3 8.7 8.8 synthesis of N-phospho-L-arginine Sabella pavonina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.3.3 5.5 7.5 pH 5.5: about 90% of maximal activity, pH 7.5: about 60% of maximal activity, synthesis of ATP Sabella pavonina
2.7.3.3 7 10 pH 7.0, about 30% of maximal activity, pH 10.0: about 35% of maximal activity, synthesis of N-phospho-L-arginine Sabella pavonina