BRENDA - Enzyme Database

L-Arginine kinase from tobacco hornworm, Manduca sexta (L.). Purification, properties, and interaction with L-canavanine

Rosenthal, G.A.; Dahlman, D.L.; Robinson, G.W.; J. Biol. Chem. 252, 3679-3683 (1977)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.45
-
MgADP-
37C
Manduca sexta
2.7.3.3
0.5
-
L-Arg
7C
Manduca sexta
2.7.3.3
0.7
-
Nomega-phospho-L-Arg
37C
Manduca sexta
2.7.3.3
22
-
L-canavanine
37C
Manduca sexta
2.7.3.3
27
-
L-phosphocanavanine
37C
Manduca sexta
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40000
-
sedimentation equilibrium centrifugation
Manduca sexta
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.3.3
ATP + L-Arg
Manduca sexta
production of high-energy reserves N-phospho-L-Arg in insect muscles
ADP + Nomega-phospho-L-Arg
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Manduca sexta
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.3.3
-
Manduca sexta
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.3.3
larva
-
Manduca sexta
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.3.3
248
-
-
Manduca sexta
Storage Stability
EC Number
Storage Stability
Organism
2.7.3.3
4C, slow loss of activity during prolonged storage, can partially be reactivated by addition of 0.1% w/v 2-mercaptoethanol
Manduca sexta
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642451
Manduca sexta
ADP + Nomega-phospho-L-Arg
-
642451
Manduca sexta
r
2.7.3.3
ATP + L-Arg
production of high-energy reserves N-phospho-L-Arg in insect muscles
642451
Manduca sexta
ADP + Nomega-phospho-L-Arg
-
-
-
?
2.7.3.3
ATP + L-canavanine
-
642451
Manduca sexta
ADP + L-phosphocanavanine
-
642451
Manduca sexta
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.3.3
monomer
1 * 40000, SDS-PAGE
Manduca sexta
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.3.3
27.3
-
L-(+)-(S)-canavanine
-
Manduca sexta
2.7.3.3
50.7
-
L-phosphocanavanine
-
Manduca sexta
2.7.3.3
139
-
L-Arg
-
Manduca sexta
2.7.3.3
431
-
Nomega-phospho-L-Arg
-
Manduca sexta
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.45
-
MgADP-
37C
Manduca sexta
2.7.3.3
0.5
-
L-Arg
7C
Manduca sexta
2.7.3.3
0.7
-
Nomega-phospho-L-Arg
37C
Manduca sexta
2.7.3.3
22
-
L-canavanine
37C
Manduca sexta
2.7.3.3
27
-
L-phosphocanavanine
37C
Manduca sexta
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40000
-
sedimentation equilibrium centrifugation
Manduca sexta
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.3.3
ATP + L-Arg
Manduca sexta
production of high-energy reserves N-phospho-L-Arg in insect muscles
ADP + Nomega-phospho-L-Arg
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
-
Manduca sexta
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.3.3
larva
-
Manduca sexta
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.3.3
248
-
-
Manduca sexta
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.3.3
4C, slow loss of activity during prolonged storage, can partially be reactivated by addition of 0.1% w/v 2-mercaptoethanol
Manduca sexta
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642451
Manduca sexta
ADP + Nomega-phospho-L-Arg
-
642451
Manduca sexta
r
2.7.3.3
ATP + L-Arg
production of high-energy reserves N-phospho-L-Arg in insect muscles
642451
Manduca sexta
ADP + Nomega-phospho-L-Arg
-
-
-
?
2.7.3.3
ATP + L-canavanine
-
642451
Manduca sexta
ADP + L-phosphocanavanine
-
642451
Manduca sexta
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.3.3
monomer
1 * 40000, SDS-PAGE
Manduca sexta
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.3.3
27.3
-
L-(+)-(S)-canavanine
-
Manduca sexta
2.7.3.3
50.7
-
L-phosphocanavanine
-
Manduca sexta
2.7.3.3
139
-
L-Arg
-
Manduca sexta
2.7.3.3
431
-
Nomega-phospho-L-Arg
-
Manduca sexta