Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Storey, K.B.
    Purification and characterization of arginine kinase from the mantle muscle of the squid, Symplectoteuthis oualaniensis. Role of the phosphagen/phosphagen kinase system in a highly aerobic muscle (1977), Arch. Biochem. Biophys., 179, 518-526.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.3.3 Ca2+
-
 Sthenoteuthis oualaniensis
2.7.3.3 Cl-
-
 Sthenoteuthis oualaniensis
2.7.3.3 Cu2+
-
 Sthenoteuthis oualaniensis
2.7.3.3 Fe2+
-
 Sthenoteuthis oualaniensis
2.7.3.3 K+ 200 mM, 50% inhibition Sthenoteuthis oualaniensis
2.7.3.3 Na+ 200 mM, 50% inhibition Sthenoteuthis oualaniensis
2.7.3.3 NADH noncompetitive Sthenoteuthis oualaniensis
2.7.3.3 NH4+ 200 mM, 50% inhibition Sthenoteuthis oualaniensis
2.7.3.3 SO42-
-
 Sthenoteuthis oualaniensis
2.7.3.3 Zn2+
-
 Sthenoteuthis oualaniensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 0.2
-
 ADP pH 7.2, 25°C Sthenoteuthis oualaniensis
2.7.3.3 0.8
-
 ATP pH 7.6, 25°C Sthenoteuthis oualaniensis
2.7.3.3 1
-
 L-Arg pH 7.6, 25°C Sthenoteuthis oualaniensis
2.7.3.3 3.5
-
 Nomega-phospho-L-Arg pH 7.2, 25°C Sthenoteuthis oualaniensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.3 Ca2+ 4% of the activation with Mg2+ Sthenoteuthis oualaniensis
2.7.3.3 Mg2+ Km: 0.6 mM Sthenoteuthis oualaniensis
2.7.3.3 Mg2+ divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mg2+: 0.6 mM Sthenoteuthis oualaniensis
2.7.3.3 Mg2+ highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate Sthenoteuthis oualaniensis
2.7.3.3 Mn2+ Km: 0.08 mM Sthenoteuthis oualaniensis
2.7.3.3 Mn2+ divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mn2+: 0.08 mM Sthenoteuthis oualaniensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 55000
-
 gel filtration Sthenoteuthis oualaniensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.3 ATP + L-Arg Sthenoteuthis oualaniensis
-
 ADP + Nomega-phospho-L-Arg
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Sthenoteuthis oualaniensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3
-
 Sthenoteuthis oualaniensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + L-Arg
-
 Sthenoteuthis oualaniensis ADP + Nomega-phospho-L-Arg
-
 ?
2.7.3.3 ATP + L-Arg
-
 Sthenoteuthis oualaniensis ADP + Nomega-phospho-L-Arg
-
 r
2.7.3.3 GDP + Nomega-phospho-L-Arg 10% of the activity with ADP Sthenoteuthis oualaniensis GTP + L-Arg
-
 ?
2.7.3.3 UDP + Nomega-phospho-L-Arg 10% of the activity with ADP Sthenoteuthis oualaniensis UTP + L-Arg
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.3 monomer 1 * 55000, SDS-PAGE Sthenoteuthis oualaniensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.3 6.3
-
 synthesis of ATP, 20 mM phosphate buffer Sthenoteuthis oualaniensis
2.7.3.3 6.9
-
 synthesis of ATP, 20 mM Tris-HCl buffer Sthenoteuthis oualaniensis
2.7.3.3 8.2
-
 synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer or 20 mM phosphate buffer Sthenoteuthis oualaniensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.3.3 5.9 6.8 pH 5.9: about 35% of maximal activity, pH 6.8: about 55% of maximal activity, synthesis of ATP, 20 mM phosphate buffer Sthenoteuthis oualaniensis
2.7.3.3 6.5 7.3 pH 6.5: about 45% of maximal activity, pH 9.0: about 45% of maximal activity, synthesis of ATP, 20 mM Tris-HCl buffer Sthenoteuthis oualaniensis
2.7.3.3 7.5 9 about 50% of maximal activity at pH 7.5 and pH 9.0, synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer Sthenoteuthis oualaniensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.3.3 0.05
-
 Zn2+
-
 Sthenoteuthis oualaniensis
2.7.3.3 0.15
-
 NADH
-
 Sthenoteuthis oualaniensis
2.7.3.3 0.5
-
 Cu2+
-
 Sthenoteuthis oualaniensis
2.7.3.3 0.85
-
 Ca2+
-
 Sthenoteuthis oualaniensis
2.7.3.3 1.5
-
 Fe2+
-
 Sthenoteuthis oualaniensis
2.7.3.3 100
-
 SO42-
-
 Sthenoteuthis oualaniensis