BRENDA - Enzyme Database

Purification and characterization of arginine kinase from the mantle muscle of the squid, Symplectoteuthis oualaniensis. Role of the phosphagen/phosphagen kinase system in a highly aerobic muscle

Storey, K.B.; Arch. Biochem. Biophys. 179, 518-526 (1977)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.3
Ca2+
-
Sthenoteuthis oualaniensis
2.7.3.3
Cl-
-
Sthenoteuthis oualaniensis
2.7.3.3
Cu2+
-
Sthenoteuthis oualaniensis
2.7.3.3
Fe2+
-
Sthenoteuthis oualaniensis
2.7.3.3
K+
200 mM, 50% inhibition
Sthenoteuthis oualaniensis
2.7.3.3
Na+
200 mM, 50% inhibition
Sthenoteuthis oualaniensis
2.7.3.3
NADH
noncompetitive
Sthenoteuthis oualaniensis
2.7.3.3
NH4+
200 mM, 50% inhibition
Sthenoteuthis oualaniensis
2.7.3.3
SO42-
-
Sthenoteuthis oualaniensis
2.7.3.3
Zn2+
-
Sthenoteuthis oualaniensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.2
-
ADP
pH 7.2, 25°C
Sthenoteuthis oualaniensis
2.7.3.3
0.8
-
ATP
pH 7.6, 25°C
Sthenoteuthis oualaniensis
2.7.3.3
1
-
L-Arg
pH 7.6, 25°C
Sthenoteuthis oualaniensis
2.7.3.3
3.5
-
Nomega-phospho-L-Arg
pH 7.2, 25°C
Sthenoteuthis oualaniensis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Ca2+
4% of the activation with Mg2+
Sthenoteuthis oualaniensis
2.7.3.3
Mg2+
divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mg2+: 0.6 mM; highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate; Km: 0.6 mM
Sthenoteuthis oualaniensis
2.7.3.3
Mn2+
divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mn2+: 0.08 mM; Km: 0.08 mM
Sthenoteuthis oualaniensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
55000
-
gel filtration
Sthenoteuthis oualaniensis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.3.3
ATP + L-Arg
Sthenoteuthis oualaniensis
-
ADP + Nomega-phospho-L-Arg
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Sthenoteuthis oualaniensis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.3.3
-
Sthenoteuthis oualaniensis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642450
Sthenoteuthis oualaniensis
ADP + Nomega-phospho-L-Arg
-
-
-
?
2.7.3.3
ATP + L-Arg
-
642450
Sthenoteuthis oualaniensis
ADP + Nomega-phospho-L-Arg
-
642450
Sthenoteuthis oualaniensis
r
2.7.3.3
GDP + Nomega-phospho-L-Arg
10% of the activity with ADP
642450
Sthenoteuthis oualaniensis
GTP + L-Arg
-
-
-
?
2.7.3.3
UDP + Nomega-phospho-L-Arg
10% of the activity with ADP
642450
Sthenoteuthis oualaniensis
UTP + L-Arg
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.3.3
monomer
1 * 55000, SDS-PAGE
Sthenoteuthis oualaniensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
6.3
-
synthesis of ATP, 20 mM phosphate buffer
Sthenoteuthis oualaniensis
2.7.3.3
6.9
-
synthesis of ATP, 20 mM Tris-HCl buffer
Sthenoteuthis oualaniensis
2.7.3.3
8.2
-
synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer or 20 mM phosphate buffer
Sthenoteuthis oualaniensis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.3.3
5.9
6.8
pH 5.9: about 35% of maximal activity, pH 6.8: about 55% of maximal activity, synthesis of ATP, 20 mM phosphate buffer
Sthenoteuthis oualaniensis
2.7.3.3
6.5
7.3
pH 6.5: about 45% of maximal activity, pH 9.0: about 45% of maximal activity, synthesis of ATP, 20 mM Tris-HCl buffer
Sthenoteuthis oualaniensis
2.7.3.3
7.5
9
about 50% of maximal activity at pH 7.5 and pH 9.0, synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer
Sthenoteuthis oualaniensis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.3.3
0.05
-
Zn2+
-
Sthenoteuthis oualaniensis
2.7.3.3
0.15
-
NADH
-
Sthenoteuthis oualaniensis
2.7.3.3
0.5
-
Cu2+
-
Sthenoteuthis oualaniensis
2.7.3.3
0.85
-
Ca2+
-
Sthenoteuthis oualaniensis
2.7.3.3
1.5
-
Fe2+
-
Sthenoteuthis oualaniensis
2.7.3.3
100
-
SO42-
-
Sthenoteuthis oualaniensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.3
Ca2+
-
Sthenoteuthis oualaniensis
2.7.3.3
Cl-
-
Sthenoteuthis oualaniensis
2.7.3.3
Cu2+
-
Sthenoteuthis oualaniensis
2.7.3.3
Fe2+
-
Sthenoteuthis oualaniensis
2.7.3.3
K+
200 mM, 50% inhibition
Sthenoteuthis oualaniensis
2.7.3.3
Na+
200 mM, 50% inhibition
Sthenoteuthis oualaniensis
2.7.3.3
NADH
noncompetitive
Sthenoteuthis oualaniensis
2.7.3.3
NH4+
200 mM, 50% inhibition
Sthenoteuthis oualaniensis
2.7.3.3
SO42-
-
Sthenoteuthis oualaniensis
2.7.3.3
Zn2+
-
Sthenoteuthis oualaniensis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.3.3
0.05
-
Zn2+
-
Sthenoteuthis oualaniensis
2.7.3.3
0.15
-
NADH
-
Sthenoteuthis oualaniensis
2.7.3.3
0.5
-
Cu2+
-
Sthenoteuthis oualaniensis
2.7.3.3
0.85
-
Ca2+
-
Sthenoteuthis oualaniensis
2.7.3.3
1.5
-
Fe2+
-
Sthenoteuthis oualaniensis
2.7.3.3
100
-
SO42-
-
Sthenoteuthis oualaniensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
0.2
-
ADP
pH 7.2, 25°C
Sthenoteuthis oualaniensis
2.7.3.3
0.8
-
ATP
pH 7.6, 25°C
Sthenoteuthis oualaniensis
2.7.3.3
1
-
L-Arg
pH 7.6, 25°C
Sthenoteuthis oualaniensis
2.7.3.3
3.5
-
Nomega-phospho-L-Arg
pH 7.2, 25°C
Sthenoteuthis oualaniensis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Ca2+
4% of the activation with Mg2+
Sthenoteuthis oualaniensis
2.7.3.3
Mg2+
divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mg2+: 0.6 mM; highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate; Km: 0.6 mM
Sthenoteuthis oualaniensis
2.7.3.3
Mn2+
divalent cation requirement is satisfied by Mg2+ or Mn2+. Km-value for Mn2+: 0.08 mM; Km: 0.08 mM
Sthenoteuthis oualaniensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
55000
-
gel filtration
Sthenoteuthis oualaniensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.3.3
ATP + L-Arg
Sthenoteuthis oualaniensis
-
ADP + Nomega-phospho-L-Arg
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
-
Sthenoteuthis oualaniensis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642450
Sthenoteuthis oualaniensis
ADP + Nomega-phospho-L-Arg
-
-
-
?
2.7.3.3
ATP + L-Arg
-
642450
Sthenoteuthis oualaniensis
ADP + Nomega-phospho-L-Arg
-
642450
Sthenoteuthis oualaniensis
r
2.7.3.3
GDP + Nomega-phospho-L-Arg
10% of the activity with ADP
642450
Sthenoteuthis oualaniensis
GTP + L-Arg
-
-
-
?
2.7.3.3
UDP + Nomega-phospho-L-Arg
10% of the activity with ADP
642450
Sthenoteuthis oualaniensis
UTP + L-Arg
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.3.3
monomer
1 * 55000, SDS-PAGE
Sthenoteuthis oualaniensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
6.3
-
synthesis of ATP, 20 mM phosphate buffer
Sthenoteuthis oualaniensis
2.7.3.3
6.9
-
synthesis of ATP, 20 mM Tris-HCl buffer
Sthenoteuthis oualaniensis
2.7.3.3
8.2
-
synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer or 20 mM phosphate buffer
Sthenoteuthis oualaniensis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.3.3
5.9
6.8
pH 5.9: about 35% of maximal activity, pH 6.8: about 55% of maximal activity, synthesis of ATP, 20 mM phosphate buffer
Sthenoteuthis oualaniensis
2.7.3.3
6.5
7.3
pH 6.5: about 45% of maximal activity, pH 9.0: about 45% of maximal activity, synthesis of ATP, 20 mM Tris-HCl buffer
Sthenoteuthis oualaniensis
2.7.3.3
7.5
9
about 50% of maximal activity at pH 7.5 and pH 9.0, synthesis of N-phospho-L-arginine, 20 mM Tris-HCl buffer
Sthenoteuthis oualaniensis