BRENDA - Enzyme Database

Purification and characterization of arginine kinase from sea-urchin eggs

Ratto, A.; Christen, R.; Eur. J. Biochem. 173, 667-674 (1988)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
1.25
-
ADP
pH 6.7, 25C
Paracentrotus lividus
2.7.3.3
2.08
-
Nomega-phospho-L-Arg
pH 6.7, 25C
Paracentrotus lividus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
42000
-
2 * 42000, SDS-PAGE
Paracentrotus lividus
2.7.3.3
81000
-
gel filtration
Paracentrotus lividus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Paracentrotus lividus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.3.3
-
Paracentrotus lividus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.3.3
egg
unfertilized
Paracentrotus lividus
-
2.7.3.3
esophagus
-
Paracentrotus lividus
-
2.7.3.3
gonad
male and female
Paracentrotus lividus
-
2.7.3.3
integument
-
Paracentrotus lividus
-
2.7.3.3
intestine
-
Paracentrotus lividus
-
2.7.3.3
lantern muscle
-
Paracentrotus lividus
-
2.7.3.3
larva
-
Paracentrotus lividus
-
2.7.3.3
mantle muscle
-
Paracentrotus lividus
-
2.7.3.3
additional information
no activity in sperm
Paracentrotus lividus
-
2.7.3.3
tube foot
-
Paracentrotus lividus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.3.3
360
-
-
Paracentrotus lividus
Storage Stability
EC Number
Storage Stability
Organism
2.7.3.3
-80C, several months
Paracentrotus lividus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642449
Paracentrotus lividus
ADP + Nomega-phospho-L-Arg
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.3.3
dimer
2 * 42000, SDS-PAGE
Paracentrotus lividus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.3.3
35
-
10 min, stable
Paracentrotus lividus
2.7.3.3
40
-
10 min, 21% loss of activity
Paracentrotus lividus
2.7.3.3
45
-
inactivation
Paracentrotus lividus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.3.3
Paracentrotus lividus
-
-
5.7
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.3
1.25
-
ADP
pH 6.7, 25C
Paracentrotus lividus
2.7.3.3
2.08
-
Nomega-phospho-L-Arg
pH 6.7, 25C
Paracentrotus lividus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
42000
-
2 * 42000, SDS-PAGE
Paracentrotus lividus
2.7.3.3
81000
-
gel filtration
Paracentrotus lividus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
-
Paracentrotus lividus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.3.3
egg
unfertilized
Paracentrotus lividus
-
2.7.3.3
esophagus
-
Paracentrotus lividus
-
2.7.3.3
gonad
male and female
Paracentrotus lividus
-
2.7.3.3
integument
-
Paracentrotus lividus
-
2.7.3.3
intestine
-
Paracentrotus lividus
-
2.7.3.3
lantern muscle
-
Paracentrotus lividus
-
2.7.3.3
larva
-
Paracentrotus lividus
-
2.7.3.3
mantle muscle
-
Paracentrotus lividus
-
2.7.3.3
additional information
no activity in sperm
Paracentrotus lividus
-
2.7.3.3
tube foot
-
Paracentrotus lividus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.7.3.3
360
-
-
Paracentrotus lividus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.3.3
-80C, several months
Paracentrotus lividus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642449
Paracentrotus lividus
ADP + Nomega-phospho-L-Arg
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.3.3
dimer
2 * 42000, SDS-PAGE
Paracentrotus lividus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.7.3.3
35
-
10 min, stable
Paracentrotus lividus
2.7.3.3
40
-
10 min, 21% loss of activity
Paracentrotus lividus
2.7.3.3
45
-
inactivation
Paracentrotus lividus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.3.3
Paracentrotus lividus
-
-
5.7